PMID: 2124676Dec 11, 1990Paper

Determination of the cis sequence involved in catabolite repression of the Bacillus subtilis gnt operon; implication of a consensus sequence in catabolite repression in the genus Bacillus

Nucleic Acids Research
Y Miwa, Y Fujita

Abstract

The mechanism underlying catabolite repression in Bacillus species remains unsolved. The gluconate (gnt) operon of Bacillus subtilis is one of the catabolic operons which is under catabolite repression. To identify the cis sequence involved in catabolite repression of the gnt operon, we performed deletion analysis of a DNA fragment carrying the gnt promoter and the gntR gene, which had been cloned into the promoter probe vector, pWP19. Deletion of the region upstream of the gnt promoter did not affect catabolite repression. Further deletion analysis of the gnt promoter and gntR coding region was carried out after restoration of promoter activity through the insertion of internal constitutive promoters of the gnt operon before the gntR gene (P2 and P3). These deletions revealed that the cis sequence involved in catabolite repression of the gnt operon is located between nucleotide positions +137 and +148. This DNA segment contains a sequence, ATTGAAAG, which may be implicated as a consensus sequence involved in catabolite repression in the genus Bacillus.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M J Weickert, G H Chambliss
Jan 1, 1986·Journal of General Microbiology·Y FujitaT Fujita
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y Fujita, T Fujita
May 15, 1973·Biochemical and Biophysical Research Communications·P Setlow
May 25, 1984·Science·B de CrombruggheH Buc

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Citations

Nov 4, 2005·Journal of Bacteriology·Graciela L LorcaMilton H Saier
Feb 10, 2009·Bioscience, Biotechnology, and Biochemistry·Yasutaro Fujita
Nov 2, 2002·The Journal of Biological Chemistry·Helena RamströmJacques Haiech
Feb 1, 1996·Microbiology·Milton H SaierJing-Jing Ye
May 1, 1992·Journal of Bacteriology·T Rygus, W Hillen
Jun 1, 1994·Applied and Environmental Microbiology·O GatY Shoham

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