Determination of the complete cDNA sequence, construction of expression systems, and elucidation of fibrinolytic activity for Tapes japonica lysozyme

Protein Expression and Purification
Kouhei TakeshitaTaiji Iomoto

Abstract

The lysozyme of the marine bivalve, Tapes japonica (13.8 kDa), belongs to the invertebrate lysozyme family and displays both chitinase and isopeptidase activities. We determined the complete cDNA sequence and constructed effective expression systems for this enzyme using Escherichia coli (BL21) and Pichia pastoris. The native and recombinant proteins indicated lysozyme activity and isopeptidase activity, including the proteolysis of d-dimer, a plasminolytic product of stabilized polymeric fibrin. These results will be utilized for the structural and functional study of invertebrate lysozymes, and for the development of applications for thrombosis therapies.

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Citations

Apr 24, 2010·Journal of Biosciences·Lien Callewaert, Chris W Michiels
May 15, 2012·Journal of Biosciences·Joris M Van Herreweghe, Chris W Michiels
Aug 5, 2009·Protein Engineering, Design & Selection : PEDS·Vida CasaiteRolandas Meskys
Nov 17, 2010·Biochemistry. Biokhimii︠a︡·L L ZavalovaI P Baskova
Jan 6, 2010·Cellular and Molecular Life Sciences : CMLS·J M Van HerrewegheC W Michiels
Aug 19, 2015·Protein Expression and Purification·Valentin A ManuveraVassili N Lazarev
May 21, 2009·Developmental and Comparative Immunology·Radka JoskováMartin Bilej
Aug 5, 2008·Bioorganicheskaia khimiia·I P Baskova, L L Zavalova
Nov 1, 2020·International Journal of Biological Macromolecules·Yoshito AbeTadashi Ueda

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