PMID: 11325422Apr 28, 2001Paper

Determination of the degree of acetylation of chitin materials by 13C CP/MAS NMR spectroscopy

International Journal of Biological Macromolecules
M L DuarteJoão Rocha

Abstract

13C CP/MAS NMR spectroscopy has been shown to be a powerful tool to quantify the degree of acetylation of chitin and chitosan. In order to optimise the parameters which afford quantitative 13C cross-polarisation magic-angle spinning NMR spectra, a detailed relaxation study has been carried out on selected chitin and deacetylated chitin samples. A relaxation delay of 5 s and a contact time of 1 ms have been found to yield quantitative NMR spectra of samples with deacetylation degree values of 0.68 and 0.16. The measured spin-lattice relaxation times in the rotating frame, T(1rhoH), are in the range 6.4-8.9 ms for chitin and 4.3-7.3 ms for deacetylated chitin, while TCH values for both samples are very similar and range from 0.03 to 0.19 ms. Spin-counting experiments indicate that, within experimental error, all carbon is detected by NMR indicating that the samples studied contain no (or very few) paramagnetic centres.

References

Aug 15, 1986·Archives of Biochemistry and Biophysics·T FukamizoG S Jacob

❮ Previous
Next ❯

Citations

Oct 1, 2013·International Journal of Biological Macromolecules·Jun LiuChang-hai Jin
Aug 9, 2003·Journal of Pharmaceutical and Biomedical Analysis·M LavertuAjay Gupta
Jun 19, 2010·Marine Drugs·Jolanta KumirskaPiotr Stepnowski
May 23, 2012·Journal of Biomedical Materials Research. Part a·Piergiorgio GentileGianluca Ciardelli
Jun 26, 2012·Journal of Tissue Engineering and Regenerative Medicine·F BoccafoschiM Cannas
Oct 20, 2012·Critical Reviews in Biotechnology·Gurpreet Singh DhillonMausam Verma
May 20, 2016·Marine Drugs·Barbara BellichAttilio Cesàro
Apr 3, 2007·International Journal of Biological Macromolecules·Douglas de Britto, Odilio B G de Assis
Apr 12, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Toshiaki Hattori, Kazuhito Katai
Jun 15, 2017·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Mara PerroneAndreas Bernkop-Schnürch
Nov 14, 2020·Carbohydrate Polymers·Mikhail V TsurkanHermann Ehrlich
Jul 24, 2021·Carbohydrate Polymers·Gaël HuetAlbert Nguyen van Nhien
Mar 9, 2004·Biomacromolecules·Géraldine Chaussard, Alain Domard
Jan 9, 2007·Biomacromolecules·Jacob D Goodrich, William T Winter

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.