PMID: 9829995Nov 26, 1998

Determination of the disulfide bonds within a B domain variant surface glycoprotein from Trypanosoma congolense

The Journal of Biological Chemistry
H BusslerE Reinwald


The disulfide bonds within a variant surface glycoprotein from Trypanosoma congolense have been determined. L-[35S]Cysteine metabolically labeled protein was digested with trypsin, and radiolabeled peptides were separated by reversed-phase high performance liquid chromatography, and putative cystine-containing peptides were subdigested with other proteases and analyzed after further purification by amino acid sequencing and mass spectrometry. All eight cysteine residues of the protein, located within the N-terminal domain, are covalently linked. The four disulfide bonds are between cysteines 16/236, 171/193, 195/206, and 286/298. This is, for the first time, the determination of disulfide bonds within a variant surface glycoprotein belonging to the B-type. As all the eight cysteines of BENat 1.3 variant surface glycoprotein are positionally conserved, the cystine pattern of this protein can be regarded as a prototype of disulfide bonding within B-type variant surface glycoproteins. Although the cysteine residues of B-type variant surface glycoproteins are located at completely different positions in the protein chain compared with A-type variant surface glycoproteins, the positions of the disulfide bonds can easily be integrate...Continue Reading


Feb 1, 1979·The Journal of protozoology·K L Banks
Mar 15, 1979·The Biochemical Journal·J G Johnson, G A Cross
May 1, 1978·The Journal of protozoology·K L Banks
Aug 1, 1978·Hoppe-Seyler's Zeitschrift für physiologische Chemie·E ReinwaldR Sälker
Dec 1, 1970·Experimental Parasitology·S M Lanham, D G Godfrey
Oct 30, 1996·Molecular and Biochemical Parasitology·M Carrington, J Boothroyd
Apr 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G A Cross


May 14, 2003·Molecular and Biochemical Parasitology·Jun WangGeorge A M Cross
Dec 13, 2000·Microbes and Infection·H TabelJ E Uzonna
Nov 30, 2011·Antioxidants & Redox Signaling·Saikh Jaharul HaqueSailen Barik
Jan 21, 2014·Protein Science : a Publication of the Protein Society·Matthew K Higgins, M Carrington
Nov 16, 2007·The Journal of Biological Chemistry·Nicola G JonesM Carrington
Nov 24, 2004·The Journal of Biological Chemistry·Anasuya ChattopadhyayM Carrington

Related Concepts

Peptide Mapping
Protein Conformation
VSG 221
Surface Enhanced Laser Desorption Ionization Mass Spectrometry

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.