Determination of the enantiomers of mefloquine in plasma and whole blood using a coupled achiral-chiral high-performance liquid chromatographic system

Journal of Chromatography
F GimenezIrving W Wainer

Abstract

A coupled achiral-chiral high-performance liquid chromatographic system has been developed for the determination of the enantiomers of mefloquine, (+)-MFQ and (-)-MFQ, in plasma and whole blood. The MFQ was separated from the interfering components in the biological matrix and quantified on a cyano-bonded phase, and the enantiomeric composition was determined on an (S)-naphthylurea chiral stationary phase. The two columns were connected by a switching valve equipped with a silica precolumn. The precolumn was used to concentrate the MFQ in the eluent from the achiral column before backflushing onto the chiral phase. The coupled-column system was validated and applied to the analysis of a pilot study of the pharmacokinetics of (+)- and (-)-MFQ in plasma and whole blood.

Citations

Jan 1, 1993·Transactions of the Royal Society of Tropical Medicine and Hygiene·L K BascoJ Le Bras
Apr 22, 1994·Journal of Chromatography. B, Biomedical Applications·L WallénU Hellgren
Dec 9, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·E SouriF Jamali
May 1, 1992·British Journal of Clinical Pharmacology·L K BascoJ Le Bras
Dec 17, 2003·Clinical Pharmacokinetics·Dion R Brocks, Reza Mehvar
Mar 21, 2007·Journal of Pharmaceutical and Biomedical Analysis·Igor Rafael dos Santos Magalhães, Pierina Sueli Bonato
Apr 18, 2002·Antimicrobial Agents and Chemotherapy·Jean M Karle, Isabella L Karle

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