Abstract
Partial structures of enterobacterial lipopolysaccharides (LPS) of the Rechemotype, consisting of lipid A and 3-deoxy-D-manno-2-octulosonic acid (Kdo), as well as oligosaccharides and derivative of Kdo were synthesized and used to characterize the epitope specificity of monoclonal antibodies against Re-mutant LPS. High-molecular-weight antigens, obtained after copolymerization of the respective allyl glycosides with acrylamide, and the haptenic oligosaccharides were used in immunoprecipitation, immune hemolysis, and in inhibition assays. A monoclonal antibody (clone 20, igM) recognizing a terminal Kdop group was shown to require for its binding the alpha-anomeric configuration and OH-4 and OH-5 groups, whereas the C-7 - C-8 chain was of minor importance. Another monoclonal antibody (clone 25, IgG3), which recognizes a (2--4)-linked Kdo disaccharide, was shown to require for its binding the alpha-anomeric configuration of both residues. The isomer having a reducing beta-Kdo residue was significantly less active, and that with a terminal beta-Kdo group was completely inactive. The OH-5 group of the reducing residue was shown to be not important for the specificity of this antibody, since it could be replaced by a hydrogen atom wi...Continue Reading
Citations
May 7, 1993·Carbohydrate Research·A HofingerH Brade
Sep 15, 1994·Carbohydrate Research·F W D'SouzaH Brade
Dec 1, 1992·Hybridoma·T PedronR Chaby
Aug 6, 1998·Bioscience, Biotechnology, and Biochemistry·X ZengK Kobayashi
Nov 1, 1991·FEMS Microbiology Immunology·T KirikaeE T Rietschel
Aug 12, 2016·PLoS Pathogens·Hendrik Koliwer-BrandlRainer Kalscheuer
Aug 25, 2001·Journal of Endotoxin Research·L BradeH Brade
Nov 4, 2000·Journal of Endotoxin Research·P KosmaH Brade
Oct 15, 1993·European Journal of Biochemistry·M KastowskyH Bradaczek
Jan 1, 1994·FEMS Immunology and Medical Microbiology·L BradeH Brade
Apr 10, 1992·Carbohydrate Research·F I AuzanneauL Szabó
Nov 26, 1999·Biochimica Et Biophysica Acta·P Kosma
Jul 1, 1992·Journal of Bacteriology·M KastowskyH Bradaczek
Oct 1, 1991·Infection and Immunity·A F VerheulJ T Poolman
Jun 1, 1994·Infection and Immunity·B J AppelmelkJ H Nuijens
Sep 1, 1997·Infection and Immunity·L BradeE T Rietschel
Mar 1, 1994·Infection and Immunity·N M van der MeerD M MacLaren
Apr 1, 1992·Infection and Immunity·Y FuH Brade
Apr 1, 1993·Immunobiology·E T RietschelS Kusumoto