Determination of the genotoxic effects of Convolvulus arvensis extracts on corn (Zea mays L.) seeds

Toxicology and Industrial Health
Serap SunarGuleray Agar

Abstract

In this research, the methanolic extracts of Convolvulus arvensis were tested for genotoxic and inhibitor activity on the total soluble protein content and the genomic template stability against corn Zea mays L. seed. The methanol extracts of leaf, stem and root of C. arvensis were diluted to 50, 75 and 100 μl concentrations and applied to corn seed. The total soluble protein and genomic template stability results were compared with the control. The results showed that especially 100 μl extracts of diluted leaf, stem and root had a strong inhibitory activity on the genomic template stability. The changes occurred in random amplification of polymorphic DNA (RAPD) profiles of C. arvensis extract treatment included variation in band intensity, loss of bands and appearance of new bands compared with control. Also, the results obtained from this study revealed that the increase in the concentrations of C. arvensis extract increased the total soluble protein content in maize. The results suggested that RAPD analysis and total protein analysis could be applied as a suitable biomarker assay for the detection of genotoxic effects of plant allelochemicals.

References

Jun 26, 2002·Physiologia Plantarum·Hisashi Kato-NoguchiShosuke Yamamura
May 10, 2003·Proceedings. Biological Sciences·Jonathan M LevineSandra Lavorel
Oct 10, 2006·Annals of Botany·Harminder P SinghRavinder K Kohli
Jan 12, 2007·Journal of Chemical Ecology·Krystyna OraczRenata Bogatek
Oct 6, 2010·Toxicology and Industrial Health·M Sinan TaspinarSevgi Sevsay

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Citations

Mar 22, 2012·Toxicology and Industrial Health·Güzin KekeçÖkkes Atici
Jul 22, 2019·Plants·Bimal Kumar GhimireIll-Min Chung

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Uvisoft
TotalLab
Bio Doc Image Analysis System
SPSS

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