Determination of the interactions between lectins and glycoproteins by surface plasmon resonance

Journal of Molecular Recognition : JMR
I OkazakiR Bhikhabhai

Abstract

A simple and rapid analytical method for detecting interactions between oligosaccharides of glycoproteins and different lectins was studied by surface plasmon resonance using a biosensor (BIAcore). The interactions of three lectins, Sambucus sieboldiana agglutinin (SSA), Ricinus communis agglutinin I (RCA I) and Concanavalin A (Con A) for fetuin and digested fetuins with glycosidases, asialo-, agalacto-, and aglucosamino-fetuin, were investigated as a model system. These fetuins were immobilized to the matrix of of the sensor chip and the lectins were injected into the sensor chip cartridge. The association and dissociation reactions could be monitored as resonance signals in real time. The interactions with lectins significantly changed as the oligosaccharides of fetuin were trimmed. The interactions between fetuin and SSA, asialofetuin and RCA I, and aglucosaminofetuin and Con A show the highest affinity properties, respectively. The association constants of these lectins were estimated to be 1.4 x 10(7), 1.9 x 10(8) and 5.3 x 10(7) (M-1), respectively. These results suggested that the interactions between lectins and glycoproteins could be well defined in real time and kinetically, and that the partial structure of oligosacc...Continue Reading

Citations

Dec 20, 1999·Analytical Biochemistry·A Satoh, I Matsumoto
May 27, 2003·Biochimie·Eric DuvergerMichel Monsigny
Sep 24, 2002·Biosensors & Bioelectronics·Mathias LiljebladPeter Påhlsson
Feb 1, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Byoung-Yun KimJong-Duk Kim
Nov 11, 1999·Journal of Molecular Recognition : JMR·M H Van Regenmortel
Jan 23, 2008·Biomacromolecules·Mieke HeydeEtienne H Schacht
Jan 21, 2000·Archives of Biochemistry and Biophysics·C J Thomas, A Surolia
Sep 26, 2002·International Journal of Biological Macromolecules·D MislovicováP Gemeiner

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