PMID: 9438449Jan 23, 1998Paper

Determination of the intracellular sodium concentration in perfused mouse liver by 31P and 23Na magnetic resonance spectroscopy

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
J M ColetA D Sherry

Abstract

A combination of 31P and 23Na NMR spectroscopy has been used to quantify the concentration of intracellular sodium, [Na]IC in the isolated and perfused mouse liver. The 31P resonances of dimethyl methylphosphonate and LaDOTP5-, markers of total tissue space and extracellular space, respectively, were used to determine the intracellular liver volume. For a mean wet weight of 1.7 +/- 0.3 g, the intracellular liver volume as measured by 31P NMR averaged 1.2 +/- 0.2 ml. The amount of intracellular sodium was measured from the baseline-resolved intracellular 23Na resonance during perfusion of the shift reagent, TmDOTP5-. These two measurements resulted in an NMR-determined value for [Na]IC of 29.0 +/- 5.2 mM. Separate measurement of total tissue Tm and Na by atomic absorption spectroscopy on the same samples provided an AAS-determined value for [Na]IC of 32.1 +/- 7.4 mM. These results indicate that intracellular sodium in the isolated, perfused liver is 100% visible by 23Na NMR spectroscopy.

References

Jul 1, 1992·Journal of Magnetic Resonance Imaging : JMRI·N BansalA D Sherry
Apr 1, 1991·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·H BlumR G Johnson
Dec 1, 1981·The Journal of Surgical Research·R L HollidayG T Shires
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Citations

Jun 17, 1999·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J M ColetA D Sherry
Mar 10, 2001·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·P M Winter, N Bansal
Apr 27, 2001·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·T Q DuongJ J Neil
Oct 15, 1998·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·N B RadfordA D Sherry
Oct 1, 2013·Biological Trace Element Research·Chunyan DaiJinchao Zhang
Jun 15, 2001·ILAR Journal·J C Chatham, S J Blackband
Feb 15, 2018·Antimicrobial Agents and Chemotherapy·Darius BabusisAdrian S Ray
Mar 20, 2021·Journal of Magnetic Resonance Imaging : JMRI·Diana M LindquistJonathan R Dillman
Mar 25, 2021·Scientific Reports·Muhammad H KhanFahmeed Hyder

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