Determination of the Isotopic Enrichment of 13 C- and 2 H-Labeled Tracers of Glucose Using High-Resolution Mass Spectrometry: Application to Dual- and Triple-Tracer Studies

Analytical Chemistry
Martin TrötzmüllerWerner Regittnig

Abstract

Multiple-tracer approaches for investigating glucose metabolism in humans usually involve the administration of stable and radioactive glucose tracers and the subsequent determination of tracer enrichments in sampled blood. When using conventional, low-resolution mass spectrometry (LRMS), the number of spectral interferences rises rapidly with the number of stable tracers employed. Thus, in LRMS, both computational effort and statistical uncertainties associated with the correction for spectral interferences limit the number of stable tracers that can be simultaneously employed (usually two). Here we show that these limitations can be overcome by applying high-resolution mass spectrometry (HRMS). The HRMS method presented is based on the use of an Orbitrap mass spectrometer operated at a mass resolution of 100 000 to allow electrospray-generated ions of the deprotonated glucose molecules to be monitored at their exact masses. The tracer enrichment determination in blood plasma is demonstrated for several triple combinations of 13C- and 2H-labeled glucose tracers (e.g., [1-2H1]-, [6,6-2H2]-, [1,6-13C2]glucose). For each combination it is shown that ions arising from 2H-labeled tracers are completely differentiated from those ari...Continue Reading

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Citations

Nov 21, 2018·Biomolecules·Alexander Triebl, Markus R Wenk
Dec 22, 2019·Current Opinion in Biotechnology·Doug K Allen, Jamey D Young
May 7, 2021·Scientific Reports·Lilian Valadares ToseFrancisco Fernandez-Lima
May 29, 2021·Current Opinion in Biotechnology·Tomasz K BednarskiJamey D Young

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