PMID: 6406017Jan 1, 1983Paper

Determination of the levels of HPr and enzyme I of the phosphoenolpyruvate-sugar phosphotransferase system in Escherichia coli and Salmonella typhimurium

Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire
R L Mattoo, E B Waygood

Abstract

The levels of histidine-containing protein HPr and enzyme I of the phosphoenolpyruvate-sugar phosphotransferase system of Escherichia coli strains 1100, NC3, W3110, and P650 and Salmonella typhimurium strains SB3507 and LJ144 have been determined by quantitative sugar phosphorylation assay and immunochemically. The levels have been determined for cells grown on minimal salts with glucose, fructose, mannitol, glycerol, and lactate and on nutrient broth. All determinations indicate a two- to three-fold change in the levels of enzyme I and HPr between growth on hexoses, which gave the higher levels, and the other growth substrates. The highest levels were not always found in glucose-grown cells. Antibodies were produced in rabbits using purified proteins from E. coli P650. The activity measurements and immunochemically determined enzyme I protein gave specific activities in the crude extracts of E. coli strains which were similar to that of the pure enzyme. The wild-type S. typhimurium enzyme I in crude extracts did not have the same immunochemical reactivity, although there was a considerable cross-reaction and the specific activity appeared to be half that of pure enzyme I. The HPr from both E. coli and S. typhimurium behaved id...Continue Reading

Citations

Jun 1, 1989·FEMS Microbiology Reviews·H De ReuseA Danchin
Jun 19, 1998·Molecular Microbiology·H AfflerbachR Wagner
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D K FoxS Garges
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·R LuxJ W Lengeler
Nov 18, 2003·Journal of Bacteriology·Harald NothaftFritz Titgemeyer
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Christoph Benning
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Oct 20, 2005·Journal of Bioscience and Bioengineering·P KotrbaH Yukawa
Jul 22, 1998·Journal of Molecular Biology·L PrasadL T Delbaere
May 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·Trang T NguyenVincenzo Venditti
Sep 1, 1985·Microbiological Reviews·P W Postma, J W Lengeler

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