PMID: 9174261Apr 11, 1997Paper

Determination of the lipid peroxidation product (E)-4-hydroxy-2-nonenal in clinical samples by gas chromatography--negative-ion chemical ionisation mass spectrometry of the O-pentafluorobenzyl oxime

Journal of Chromatography. B, Biomedical Sciences and Applications
M L Selley

Abstract

(E)-4-Hydroxy-2-nonenal (HNE) is a highly reactive product of the free radical-stimulated lipid peroxidation of phospholipid-bound arachidonic acid in cellular membranes. We describe a sensitive and specific method for the determination of HNE in clinical samples. The method is based on the formation of the O-pentafluorobenzyl (O-PFB) oxime derivative of HNE, which is then extracted and cleaned up by solid-phase extraction. The HNE O-PFB oxime is then analysed without further derivatisation by capillary column gas chromatography-negative ion chemical ionisation mass spectrometry (GC-NICI-MS) using selected-ion monitoring. Concentrations down to the pmol range were achieved using deuterated HNE as an internal standard. The method was used to determine HNE in the cerebrospinal fluid and plasma of patients with Parkinson's disease, the plasma of patients with HIV-1 infection and AIDS and in inflamed mucosal biopsy specimens from patients with inflammatory bowel disease.

References

Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·S ToyokuniE R Stadtman

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Citations

Oct 22, 2011·Analytical and Bioanalytical Chemistry·Magdalena LigorBogusław Buszewski
Feb 12, 2002·Lipids·D Praticò
Jul 17, 1998·Free Radical Biology & Medicine·M L Selley
Jan 16, 1999·Free Radical Biology & Medicine·L L de ZwartN P Vermeulen
Jun 22, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dorothee Spies-MartinMichael Leichsenring
Nov 28, 2002·Alimentary Pharmacology & Therapeutics·L Kruidenier, H W Verspaget
Feb 3, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shinji TsunoiMinoru Tanaka
Mar 5, 2015·Chemical Research in Toxicology·Christopher D AluiseLawrence J Marnett
Feb 20, 2015·Naunyn-Schmiedeberg's Archives of Pharmacology·Asami MoriKunio Ishii
Jun 9, 2009·Free Radical Biology & Medicine·Etsuo Niki
Apr 25, 2006·Experimental Eye Research·Agnieszka PladzykSatish K Srivastava
Jan 17, 2012·Free Radical Biology & Medicine·Qingling LiGuo-Fang Zhang
Jul 16, 2016·Circulation Research·Kathy K GriendlingUNKNOWN American Heart Association Council on Basic Cardiovascular Sciences
Dec 7, 2007·Medicinal Research Reviews·G PoliG Leonarduzzi
Jan 11, 2001·Current Opinion in Clinical Nutrition and Metabolic Care·P ThérondA Legrand
Mar 11, 2021·Oxidative Medicine and Cellular Longevity·Zhenjia YaoGaiqing Wang
Sep 5, 2002·Journal of Agricultural and Food Chemistry·Emanuela ZanardiRoberto Chizzolini

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