Determination of the Major Component of Water Fraction of Katuk (Sauropus androgynous (L.) Merr.) Leaves by Liquid Chromatography-Mass Spectrometry

Journal of Pharmacy & Bioallied Sciences
Resmi MustarichieYoppi Iskandar

Abstract

The katuk leaf (Sauropus androgynous (L.) Merr.) is one of the plants that are used to overcome baldness by the people of Kampung Mak Kemas, Malaysia. It is suspected that secondary metabolites contained in katuk leaves play a key role in stimulating hair growth. The aim of this study was to identify the optimum method to obtain one of the chemical compounds in the water fraction and to identify the hypothesized chemical isolates in the water fraction katuk leave's ethanol extract. The methods used in this study included the collection and determination of the katuk plant, the processing of the katuk, phytochemical filtrating, extracting with ethanol 96%, and fractionation using the liquid-liquid extraction method with n-hexane, ethyl acetate, and water solvents The water fraction of katuk leaves was analyzed by its components by thin-layer chromatography using the stationary phase of silica gel 60 F254, developer of n-butanol:acetic acid:water (4:1:5), and detection under ultraviolet (UV) light at a wavelength of 366 and 254nm, as well as with vanillin-sulfuric acid reagent. To isolate the compounds from water fraction of katuk leaves, it was then eluted with a vacuum column chromatography by eluent with a level polarity that ...Continue Reading

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