PMID: 11320505Apr 26, 2001Paper

Determination of the maximum specific uptake capacities for glucose and oxygen in glucose-limited fed-batch cultivations of Escherichia coli

Biotechnology and Bioengineering
H Y LinP Neubauer

Abstract

A simple pulse-based method for the determination of the maximum uptake capacities for glucose and oxygen in glucose limited cultivations of E. coli is presented. The method does not depend on the time-consuming analysis of glucose or acetate, and therefore can be used to control the feed rate in glucose limited cultivations, such as fed-batch processes. The application of this method in fed-batch processes of E. coli showed that the uptake capacity for neither glucose nor oxygen is a constant parameter, as often is assumed in fed-batch models. The glucose uptake capacity decreased significantly when the specific growth rate decreased below 0.15 h(-1) and fell to about 0.6 mmol g(-1) h(-1) (mmol per g cell dry weight and hour) at the end of fed-batch fermentations, where specific growth rate was approximately 0.02 h(-1). The oxygen uptake capacity started to decrease somewhat earlier when specific growth rate declined below 0.25 h(-1) and was 5 mmol g(-1) h(-1) at the end of the fermentations. The behavior of both uptake systems is integrated in a dynamic model which allows a better fitting of experimental values for glucose in fed-batch processes in comparison to generally used unstructured kinetic models.

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Citations

Jul 8, 2005·Applied Microbiology and Biotechnology·F R Schmidt
Mar 1, 2012·Applied Microbiology and Biotechnology·Sirichai SunyaNathalie Gorret
Sep 9, 2011·Journal of Industrial Microbiology & Biotechnology·Hendrik Waegeman, Wim Soetaert
Jun 18, 2002·Journal of Biotechnology·Kai W Wirtz
Jun 19, 2013·Applied and Environmental Microbiology·Timothy E RiedelSteven E Finkel
Oct 23, 2008·Microbial Cell Factories·Jaakko SoiniPeter Neubauer
Nov 20, 2008·Microbial Cell Factories·Johanna Panula-PeräläPeter Neubauer
Aug 2, 2007·Journal of Industrial Microbiology & Biotechnology·Marjan De MeyErick Vandamme
Mar 10, 2017·ACS Synthetic Biology·William BothfeldKeith E J Tyo
Jan 26, 2017·Biotechnology Journal·Wieland N ReicheltChristoph Herwig
Mar 31, 2004·Biotechnology and Bioengineering·Ross Carlson, Friedrich Srienc
Oct 4, 2016·Biotechnology and Bioengineering·M N Cruz BournazouP Neubauer
Sep 29, 2011·Biotechnology Progress·Patrick Wechselberger, Christoph Herwig
Jul 19, 2019·Biotechnology and Bioengineering·Emmanuel AnaneMariano Nicolas Cruz Bournazou
Jan 7, 2005·Biotechnology and Bioengineering·James Zawada, James Swartz
Apr 14, 2020·PLoS Computational Biology·Marco MauriEugenio Cinquemani
Jan 19, 2018·Engineering in Life Sciences·Sebastian WeberChristian Dietzsch
Nov 14, 2016·Engineering in Life Sciences·David Benjamin NickelAndreas Knepper
Oct 30, 2020·Frontiers in Microbiology·Fabrizio CarteniStefano Mazzoleni

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