Determination of the methylation status of MGMT in different regions within glioblastoma multiforme.

Journal of Neuro-oncology
Mark G HamiltonJacob C Easaw

Abstract

Epigenetic silencing of the MGMT gene through promoter methylation correlates with improved survival in Glioblastoma Multiforme (GBM) patients receiving concurrent chemoradiotherapy. Although the clinical benefit is primarily seen in patients with methylated MGMT promoter, some unmethylated patients also respond to Temozolomide. One possible explanation may be intratumoral heterogeneity. This study was designed to assess the methylation status of the MGMT promoter in different areas of GBM and determine if methylation status varied depending on the fixation technique (paraffin-embedding versus fresh frozen) used to store tissue. Using intraoperative navigation, biopsies were obtained from three distinct regions: the enhancing outer area, the non-enhancing inner core, and an area immediately outside the enhancing region. Only patients with GBM were included for evaluation and analysis. Samples taken from each area were divided with half stored by flash freezing and the other half stored using paraffin fixation. Methylation Specific-PCR (MS-PCR) was used for analysis of MGMT promoter methylation. Thirteen patients were included. Ten were male with a median age of 62 years. In each patient, samples were taken from the enhancing ri...Continue Reading

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Citations

Feb 10, 2016·Scientific Reports·Rui-Wei MengMei-Xia Lu
Mar 25, 2018·Practical Neurology·Zhaleh KhaleeliGordon T Plant
Feb 12, 2011·Journal of Neuro-oncology·Fateme SalehiRicardo V Lloyd
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