Determination of the minimum fully protective dose of adenovirus-based DIVA vaccine against peste des petits ruminants virus challenge in East African goats

Veterinary Research
Barbara HolzerMichael D Baron

Abstract

Peste des petits ruminants virus (PPRV) causes an economically important disease of sheep and goats, primarily in developing countries. It is becoming the object of intensive international control efforts. Current vaccines do not allow vaccinated and infected animals to be distinguished (no DIVA capability). We have previously shown that recombinant, replication-defective, adenovirus expressing the PPRV H glycoprotein (AdH) gives full protection against wild type PPRV challenge. We have now tested lower doses of the vaccine, as well as AdH in combination with a similar construct expressing the PPRV F glycoprotein (AdF). We show here that, in a local breed of goat in a country where PPR disease is common (Kenya), as little as 10(7) pfu of AdH gives significant protection against PPRV challenge, while a vaccine consisting of 10(8) pfu of each of AdH and AdF gives apparently sterile protection. These findings underline the utility of these constructs as DIVA vaccines for use in PPR control.

References

Aug 18, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P MukherjeeS Rath
Mar 4, 2003·Nature Reviews. Molecular Cell Biology·Lars Ellgaard, Ari Helenius
Aug 1, 1964·Gut·S K LAI
Jul 14, 2010·Expert Review of Vaccines·Arnab SenRaj Kumar Singh
Sep 17, 2010·The Journal of General Virology·Ashley C BanyardGenevieve Libeau
Dec 4, 2010·Journal of Virological Methods·Carrie A BattenTom Barrett
Jul 5, 2011·The Veterinary Record·M D BaronC A L Oura
Jan 15, 2013·Veterinary Microbiology·Emmanuel AlbinaGeneviève Libeau
Feb 25, 2014·Veterinary Research·Jana BaronMichael D Baron

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Citations

May 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeffrey C MarinerPeter L Roeder
Feb 9, 2020·Microbial Pathogenesis·Xue-Xia JiaZhen-Chuan Fan
Aug 7, 2021·EFSA Journal·UNKNOWN EFSA Panel on Animal Health and Welfare (AHAW)Yves Van der Stede
Oct 2, 2021·Frontiers in Immunology·Daniel Rodríguez-MartínVerónica Martín
Sep 28, 2021·Frontiers in Veterinary Science·José M RojasVerónica Martín

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Methods Mentioned

BETA
PCR

Software Mentioned

R package multcomp
nlme
R

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