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Determination of the molar absorptivity of NADH

Clinical Chemistry

Feb 1, 1976

Robert McCombGeorge N Bowers

PMID: 2388

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Abstract

The molar absorptivity of NADH at 340 nm has been determined by an indirect procedure in which high-purity glucose is phosphorylated by ATP in the presence of hexokinase, coupled to oxidation of the glucose-6-phosphate by NAD+ in the presence of glucose-6-phosphate dehydrogenase. The av...read more

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  • Determination of the molar absorptivity of NADH

    Clinical Chemistry

    Feb 1, 1976

    Robert McCombGeorge N Bowers

    PMID: 2388

    DOI:

    Abstract

    The molar absorptivity of NADH at 340 nm has been determined by an indirect procedure in which high-purity glucose is phosphorylated by ATP in the presence of hexokinase, coupled to oxidation of the glucose-6-phosphate by NAD+ in the presence of glucose-6-phosphate dehydrogenase. The av...read more

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