Determination of the novel non-steroidal anti-inflammatory drug lornoxicam and its main metabolite in plasma and synovial fluid

Journal of Chromatography. B, Biomedical Sciences and Applications
S Radhofer-Welte, P Dittrich

Abstract

A rapid and sensitive HPLC method for the determination of the non-steroidal anti-inflammatory drug lornoxicam in plasma samples of humans and laboratory animals is described. After addition of the internal standard (tenoxicam) the plasma sample is acidified and extracted either by dichloromethane via Extrelut columns or by solid-phase extraction using C18 columns. After evaporation of the solvent the separation is performed on a C18 column in isocratic mode with a mobile phase consisting of 0.1 M phosphate buffer (pH 6.0)-methanol and detection at 372 nm. The limit of determination was set to 10 ng/ml using 0.5 ml of sample but can be extended down to 2.0 ng/ml plasma. Using solid-phase extraction with C18 columns both lornoxicam and its main metabolite 5'-hydroxylornoxicam can be determined while extraction via Extrelut was used in studies where only lornoxicam was to be determined. This method was used successfully in several thousand samples of pharmacokinetic and bioavailability studies in animals and in humans.

References

Sep 9, 1988·Journal of Chromatography·F D Boudinot, S S Ibrahim
Jan 10, 1986·Journal of Chromatography·P HeizmannK Zinapold
May 1, 1995·Journal of Pharmaceutical and Biomedical Analysis·L EdnoM Galtier
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Citations

Apr 2, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Robert Roskar, Vojko Kmetec
Mar 5, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·M M GhoneimA Radi
Sep 27, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ya-Li LiuHong-Hao Zhou
Jul 16, 2011·Journal of Pharmaceutical and Biomedical Analysis·Dattatray T ModhaveSaranjit Singh
Oct 22, 2016·Drug Development and Industrial Pharmacy·Soad Ali YehiaMary Yosry Aziz

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