PMID: 6165869Apr 1, 1981Paper

Determination of the nuclear RNA content of cells from donors of three different ages during in vitro senescence

Mechanisms of Ageing and Development
P BemillerA J Pappelis

Abstract

Diploid fibroblasts from male donors aged 8, 40 and 84 years were grown in vitro and their nuclear RNA content was determined at early and late doublings by absorption cytophotometry after staining with Azure B for 2 hours at 40 degree C and pH 4. It was found that the average nuclear RNA content of the early doublings was lower than that of the last doubling in all three strains. This difference became smaller as donor age increased. Also, as the age of the donor increased the percentage of early passage cells with a relative nuclear RNA content of less than 6 arbitrary units decreased, those with a relative nuclear RNA content of more than 6 units increased and the diversity in nuclear RNA content increased. The distribution pattern of relative RNA content for late passage cells was similar for the three donors.

References

Apr 1, 1979·Mechanisms of Ageing and Development·P M Bemiller, J E Miller
Jun 1, 1978·Mechanisms of Ageing and Development·S C LeeA J Pappelis
Dec 1, 1978·Mechanisms of Ageing and Development·P M Bemiller, L H Lee
Sep 1, 1976·Experimental Cell Research·P D BowmanC W Daniel
Feb 1, 1975·Experimental Cell Research·J M Ryan, V J Cristofalo
Mar 1, 1976·Experimental Cell Research·M R Klass, J Smith-Sonneborn
Jul 1, 1974·The Journal of Cell Biology·J R Smith, L Hayflick
Jun 1, 1970·Experimental Cell Research·J R Nilsson, V Leick
Jan 1, 1971·The Journal of Cell Biology·B Satir, E R Dirksen
Jun 1, 1966·Experimental Cell Research·A Macieira-CoelhoL Philipson
Jun 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·E H MCCONKEY, J W HOPKINS
Sep 1, 1965·The Journal of Cell Biology·I L Cameron, E E Guile

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Citations

Mar 1, 1987·Mechanisms of Ageing and Development·B M Stanulis-Praeger

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