Determination of the reducing terminal sugars of glycosaminoglycans using 2-aminopyridine

Journal of Biochemical and Biophysical Methods
K TakagakiM Endo

Abstract

The fluorescence labeling method (Takemoto, H. et al. (1985) Anal. Biochem. 145, 245-250) has been shown to have high sensitivity for measuring the sugar composition of glycoproteins. In the present study, its applicability for analysis of the reducing terminal sugars of glycosaminoglycans was investigated. The procedure involved coupling of glycosaminoglycans with 2-aminopyridine, followed by hydrolysis and N-acetylation, and then analysis by high-performance liquid chromatography on a reverse-phase column. The method was found to be useful for simultaneous determination of acidic, neutral and amino sugars at the reducing termini of glycosaminoglycan moieties.

References

Nov 14, 1978·Biochemical and Biophysical Research Communications·S HaseY Matsushima
Jan 1, 1979·Journal of Biochemistry·S HaseY Matsushima
Mar 19, 1987·Biochimica Et Biophysica Acta·H Matsue, M Endo
Jan 17, 1984·Biochemistry·W KnudsonH E Conrad
Jul 1, 1984·Journal of Biochemical and Biophysical Methods·M MajimaM Endo

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Citations

Jun 1, 1994·Journal of Biochemical and Biophysical Methods·K TakagakiM Endo
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·B E RothenbergA Varki
Apr 12, 2003·The Tohoku Journal of Experimental Medicine·Shigeru ShibataMasahiko Endo
Dec 1, 1993·Glycoconjugate Journal·K KuboM Endo
Jan 20, 2007·Biomedical Chromatography : BMC·Shinsuke InagakiToshimasa Toyo'oka
Mar 6, 2002·The Journal of Biological Chemistry·Keiichi TakagakiMasahiko Endo

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