Determination of the stability of protein pools from the cell wall of fungi

Research in Microbiology
José Ruiz-HerreraR Sentandreu

Abstract

Stability of the protein populations present in the cell wall of three ascomycetous fungi Candida albicans, Saccharomyces cerevisiae and Yarrowia lipolytica was investigated. Cell wall proteins were either labeled with biotin or radiolabeled with amino acids, and chased for a period of time representing several generations. Proteins linked by non-covalent or covalent bonds were separated and their turnover was analyzed. No significant turnover took place during the chase period, and in fact radioactive proteins were accumulated in the wall during the period possibly by transfer through the secretory pathway. This transfer did not involve de novo protein synthesis; it was inhibited by azide, and by incubation of a sec1 mutant of S. cerevisiae at the non-permissive temperature. It is concluded that proteins bound to the cell wall are stable and that there is no precursor-product relationship among those linked by non-covalent bonds and the covalently bound ones.

Citations

Aug 29, 2003·Phytochemistry·D M RastG O Hollenstein
Dec 26, 2006·Eukaryotic Cell·Mathias L Richard, Armêl Plaine
Aug 25, 2011·Future Microbiology·Frans M KlisStanley Brul
Jan 1, 2010·Yeast·Frans M KlisPiet W J de Groot
Oct 18, 2006·FEMS Yeast Research·S Bartnicki-Garcia
Jan 16, 2007·Environmental Monitoring and Assessment·Asha B Chelani, Sukumar Devotta
Jul 31, 2019·Applied Microbiology and Biotechnology·Jesús Urbar-UlloaJorge Verdín

Related Concepts

Cochliobolus
Medebiotin
Cell Wall
Yeast Proteins

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