Determination of the structure and degree of polymerisation of fructans from Echinacea purpurea roots

Carbohydrate Research
Maren Wack, Wolfgang Blaschek

Abstract

Highly water soluble fructans have been isolated from Echinacea purpurea (L.) Moench. roots by hot water extraction and precipitation at three different ethanol concentrations (80% v/v, 60% v/v and 40% v/v). The structure of the fructans has been characterised by three analytical methods: GC of silylated oxime derivatives and partially methylated alditol acetates, respectively, as well as 13C NMR analysis. The mean degree of polymerisation (mean DP) of each fructan has been determined by the glucose/fructose ratio. E. purpurea fructans represent linear inulin-type fructans with almost exclusively beta-(2-->1)-linked fructosyl units, terminal glucose and terminal fructose. Small proportions of beta-(2-->1,2-->6)-linked branch point residues were detected. The mean DP of the fructan fractions depends on the ethanol concentration used for precipitation: the lower the ethanol concentration the higher the mean DP. Corresponding results were found with all of the three analytical methods: 80% ethanol-insoluble fructan from E. purpurea shows an average mean DP of 35, 60% ethanol-insoluble fructan of 44 and 40% ethanol-insoluble fructan of 55. The applied methods provide sufficient sensitivity to determine not only the composition and ...Continue Reading

Citations

Aug 31, 2013·Journal of Chromatography. a·Noureddine Benkeblia
Nov 12, 2013·International Journal of Biological Macromolecules·L Moreno-ViletD P Portales-Perez
May 26, 2012·TheScientificWorldJournal·Christian Michel-CuelloMiguel Ruiz-Cabrera
Mar 26, 2015·Plant Foods for Human Nutrition·Bang-orn SrinamebSupalax Srijaranai
Jun 5, 2016·Carbohydrate Polymers·Ana Georgina Oliveira PontesMary Anne Medeiros Bandeira
Dec 23, 2015·Journal of the Science of Food and Agriculture·Emmanuel Flores-GirónEmma Gloria Ramos-Ramírez
Oct 19, 2019·Biomolecules·Erin DobrangeWim Van den Ende
Jan 1, 2020·Carbohydrate Polymers·Raluca NastaseIsabelle Capron
Aug 28, 2020·Food Research International·Pedro Felipe Pereira ChavesLucimara Mach Côrtes Cordeiro
Apr 8, 2009·Journal of Agricultural and Food Chemistry·Neil RavenscroftElizabeth M Timme
Jul 11, 2021·International Journal of Biological Macromolecules·Carlos M N MendonçaRicardo P S Oliveira

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