Determination of the structure of an endoglucanase from Aspergillus niger and its mode of inhibition by palladium chloride

Acta Crystallographica. Section D, Biological Crystallography
Shahram KhademiEdgar F Meyer

Abstract

The fungus Aspergillus niger is a main source of industrial cellulase. beta-1,4-Endoglucanase is the major component of cellulase from A. niger. In spite of widespread applications, little is known about the structure of this enzyme. Here, the structure of beta-1,4-endoglucanase from A. niger (EglA) was determined at 2.1 A resolution. Although there is a low sequence identity between EglA and CelB2, another member of family 12, the three-dimensional structures of their core regions are quite similar. The structural differences are mostly found in the loop regions, where CelB2 has an extra beta-sheet (beta-sheet C) at the non-reducing end of the binding cleft of the native enzyme. Incubation of EglA with PdCl(2) irreversibly inhibits the EglA activity. Structural studies of the enzyme-palladium complex show that three Pd(2+) ions bind to each EglA molecule. One of the Pd(2+) ions forms a coordinate covalent bond with Met118 S(delta) and the nucleophilic Glu116 O(epsilon1) at the active site of the enzyme. The other two Pd(2+) ions bind on the surface of the protein. Binding of Pd(2+) ions to EglA does not change the general conformation of the backbone of the protein significantly. Based on this structural study, one can conclud...Continue Reading

Citations

Feb 12, 2009·Applied Microbiology and Biotechnology·Hikaru NakazawaYasushi Morikawa
Dec 18, 2004·Biotechnology Letters·Lars Hildén, Gunnar Johansson
Mar 24, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Marcelo Vizoná LiberatoIgor Polikarpov
Jul 18, 2012·BMC Genomics·Mikael R AndersenJens Nielsen
Jun 10, 2009·Colloids and Surfaces. B, Biointerfaces·Ali Asghar RastegariAsghar Taheri-Kafrani
Nov 25, 2003·Protein Science : a Publication of the Protein Society·Mats SandgrenColin Mitchinson
Mar 1, 2014·Biotechnology and Bioengineering·André R L DamásioFabio M Squina
Jan 22, 2015·Applied Microbiology and Biotechnology·Gilvan P FurtadoRichard J Ward
Apr 8, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Yun ChenRey Ting Guo
Oct 31, 2012·International Journal of Biological Macromolecules·Afshin IramAabgeena Naeem
Nov 28, 2014·Microbiology and Molecular Biology Reviews : MMBR·Fernando SegatoRolf A Prade
May 8, 2016·Biochemical and Biophysical Research Communications·Junjie YanRey-Ting Guo
Mar 19, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Fernando SegatoIgor Polikarpov
Jun 14, 2005·Progress in Biophysics and Molecular Biology·Mats SandgrenColin Mitchinson
Jan 30, 2015·Chemical Reviews·Christina M PayneGregg T Beckham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.