Determination of the surface pK of carboxylic- and amine-terminated alkanethiols using surface plasmon resonance spectroscopy

Langmuir : the ACS Journal of Surfaces and Colloids
Kenan P FearsRobert A Latour

Abstract

When using self-assembled monolayers (SAMs) with ionizable functional groups, such as COOH and NH2, the dissociation constant (pKd) of the surface is an important property to know, since it defines the charge density of the surface for a given bulk solution pH. In this study, we developed a method using surface plasmon resonance (SPR) spectroscopy for the direct measurement of the pKd of a SAM surface by combining the ability of SPR to detect the change in mass concentration close to a surface and the shift in ion concentration over the surface as a function of surface charge density. This method was then applied to measure the pKd values of both COOH- and NH2-functionalized SAM surfaces using solutions of CsCl and NaBr salts, respectively, which provided pKd values of 7.4 and 6.5, respectively, based on the bulk solution pH. An analytical study was also performed to theoretically predict the shape of the SPR plots by calculating the excess mass of salt ions over a surface as a function of the difference between the solution pH and surface pKd. The analytical relationships show that the state of surface charge also influences the local hydrogen ion concentration, thus resulting in a substantial local shift in pH at the surface ...Continue Reading

References

Feb 19, 2000·Current Opinion in Biotechnology·R L Rich, D G Myszka
Aug 6, 2003·Journal of Pharmaceutical and Biomedical Analysis·Thierry LivacheYves Levy
Oct 21, 2004·Analytical Biochemistry·Géraldine FerracciChristian Lévêque
Dec 2, 2004·Journal of Biochemical and Biophysical Methods·Christine CampagnoloCarl A Batt
May 10, 2005·Biosensors & Bioelectronics·Anand SubramanianThomas Ryan
Apr 6, 2006·Chemistry and Physics of Lipids·Mojca BesenicarGregor Anderluh

❮ Previous
Next ❯

Citations

Jun 7, 2012·Analytical Chemistry·Antonio M LuqueRafael Andreu
Jul 29, 2010·Journal of the American Chemical Society·Eric A Josephs, Tao Ye
Oct 2, 2013·Journal of the American Chemical Society·Kenan P FearsDmitri Y Petrovykh
Jan 8, 2009·Journal of the American Chemical Society·Hyunsook JungPaul S Cremer
Sep 21, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Nicolas R ChevalierPatrick Guenoun
Mar 9, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Aby A ThyparambilRobert A Latour
May 13, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Yang Wei, Robert A Latour
Jun 9, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Kenan P Fears, Robert A Latour
Jul 21, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Kenan P FearsRobert A Latour
Nov 7, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Julia S ApteDavid G Castner
Mar 13, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Nadeem A VelloreSteven J Stuart
Jan 9, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Eric R DionneAntonella Badia
Mar 4, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Fang ChengJing-Ping Qu
Oct 3, 2015·Acta Biomaterialia·Matthew N RushElizabeth L Hedberg-Dirk
Feb 9, 2016·Interface Focus·Lewis MartinSerdar Kuyucak
Feb 18, 2010·Journal of Electron Spectroscopy and Related Phenomena·J E BaioDavid G Castner
Mar 15, 2016·Journal of the American Chemical Society·Jae Jin BangShelley A Claridge
Jul 20, 2011·Biotechnology and Bioengineering·Nuno Miranda CoelhoGeorge Altankov
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
May 21, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Plinio MaroniAlberto Tiraferri
Dec 17, 2011·Journal of Colloid and Interface Science·Jialong ChenNan Huang
Sep 25, 2012·Physical Chemistry Chemical Physics : PCCP·Daiana A CapdevilaDaniel H Murgida
May 18, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·K G Sprenger, Jim Pfaendtner
Sep 16, 2016·Journal of Colloid and Interface Science·Zahra BesharatMark W Rutland
May 28, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Michael Deighan, Jim Pfaendtner
Mar 6, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Tillmann UteschMaria Andrea Mroginski
Apr 9, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Waldemar A MarmisolléDaniel H Murgida
Dec 16, 2016·ACS Applied Materials & Interfaces·Marine BrunetFrançois Ozanam
Feb 9, 2017·Journal of the American Chemical Society·Simona StrazdaiteHuib J Bakker
Dec 21, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Smita Mukherjee, Alokmay Datta
Jan 12, 2020·Nature Communications·Nayoung KimMolly M Stevens
Jan 1, 2021·Chemosphere·Alireza FathollahiAlan P Newman
Apr 12, 2021·Journal of Colloid and Interface Science·Laura H JohnLuke A Clifton
Mar 7, 2018·The Journal of Physical Chemistry Letters·Mathew S ThomasC Daniel Frisbie

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.