Determination of the vitronectin binding site on plasminogen activator inhibitor 1 (PAI-1)

FEBS Letters
M van MeijerH Pannekoek

Abstract

Vitronectin is the carrier protein of plasminogen activator inhibitor 1 (PAI-1). We used a well-characterized panel of anti-human PAI-1 monoclonal antibodies (MoAbs) to localize the vitronectin-binding site on PAI-1. By employing a direct vitronectin/PAI-1 binding assay and two vitronectin-dependent inhibition assays, we demonstrate that the anti-PAI-1 MoAbs CLB-5, CLB-10, CLB-2C8 and I1, directed against different epitopes in the region between amino acids 110 and 145, prevent the interaction of PAI-1 with vitronectin. We conclude that the region between amino acids 110 and 145 of PAI-1 harbours an important determinant for the interaction with vitronectin.

References

Jan 16, 1992·Nature·J MottonenE J Goldsmith
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T NyD J Loskutoff
Dec 1, 1986·The Journal of Clinical Investigation·D GinsburgT D Gelehrter
Oct 15, 1985·The Biochemical Journal·K T PreissnerG Müller-Berghaus
Jan 1, 1983·Annual Review of Biochemistry·J Travis, G S Salvesen

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Citations

Dec 1, 1995·Trends in Biotechnology·R A EnghA J Schulze
Dec 31, 2003·Methods : a Companion to Methods in Enzymology·Philip A PatstonSteven T Olson
Jan 11, 2002·European Journal of Biochemistry·Nuria Arroyo De PradaViktor Magdolen
Jul 29, 2008·The Journal of Biological Chemistry·Christine R ScharCynthia B Peterson
Dec 22, 1999·Obstetrical & Gynecological Survey·C J Lockwood
May 1, 1997·Expert Opinion on Investigational Drugs·P Charlton
Apr 15, 2000·The Journal of Biological Chemistry·T J PodorJ I Weitz
Oct 3, 2006·Journal of Thrombosis and Haemostasis : JTH·C E LeikD A Lawrence
Feb 20, 2014·Medicinal Research Reviews·Ahmed H MekkawyDavid L Morris
Apr 8, 2015·PloS One·Karl C DeschDavid Ginsburg
Mar 16, 2004·British Journal of Haematology·Anton J G Horrevoets
Jan 9, 2021·Frontiers in Cardiovascular Medicine·Machteld Sillen, Paul J Declerck

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