Determination of theophylline and its metabolites by liquid chromatography

Journal of Chromatographic Science
R K DesirajuR L Mayock


A high pressure liquid chromatographic assay has been developed for the separation and quantitation of theophylline and its metabolites (1-methyl uric acid, 3-methyl xanthine, and 1,3-dimethyl uric acid), theobromine, and dyphylline in biological fluids, viz. human serum, urine, and saliva. The serum has been rendered protein-free by passage through a filter with a nominal molecular weight limit of 10,000. The filtrate is injected onto a reverse-phase column and the separation achieved by utilizing a polar mobile phase and dyphylline (dihydroxypropyl theophylline) as the internal standard. The results for two normal subjects are included to illustrate the applicability of the technique.


Jan 1, 1981·European Journal of Clinical Pharmacology·J H JonkmanS Riegelman
Jan 1, 1986·Journal of Pharmaceutical and Biomedical Analysis·N DaoudK G Wahlund
Aug 1, 1990·Pharmacology & Toxicology·J E Nielsen-KudskC Siggaard
Jun 20, 2018·The American Journal of Clinical Nutrition·Casey M RebholzJosef Coresh

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High Pressure Liquid Chromatography Procedure

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