Determination of thiocyanate in exhaled breath condensate

Free Radical Biology & Medicine
Joshua D ChandlerDean P Jones

Abstract

Thiocyanate is a heme peroxidase substrate that scavenges oxidants produced during inflammation and regulates host defense. In cystic fibrosis (CF) patients, increased airway thiocyanate levels are associated with improved lung function. Research on airway thiocyanate is limited, however, because convenient non-invasive airway sampling methods, such as exhaled breath condensate (EBC), yield low concentrations that are difficult to detect with available assays. In the present study, we developed a method for the determination of thiocyanate in dilute samples using isotope dilution headspace gas chromatography-coupled high-resolution, accurate-mass mass spectrometry (GC-HRMS). The method reliably quantified as little as 4 pmol thiocyanate in EBC and could detect even lower amounts. We successfully measured thiocyanate in EBC from seven healthy donors, with a mean ± SD of 27 ± 16 nM and a median inter-assay coefficient of variation of 10.4% over six months. The method was applied to other biological fluids (plasma from the same visit as EBC donation; bronchoalveolar lavage fluid [BALF] from infants with CF; and healthy adult mouse BALF), giving reliable quantification of samples ranging from 10 nM to 100 µM. Thiocyanate concentrat...Continue Reading

References

Jun 1, 1987·The American Review of Respiratory Disease·T W MarcyH Y Reynolds
May 1, 1985·The American Journal of Clinical Nutrition·P A DahlbergO Claesson
Mar 8, 2000·The European Respiratory Journal·C WardE H Walters
Mar 11, 2003·American Journal of Respiratory Cell and Molecular Biology·Corinne Wijkstrom-FreiGregory E Conner
Sep 4, 2004·The Journal of Physiology·Miryam A FragosoGregory E Conner
Sep 2, 2005·The European Respiratory Journal·I HorváthUNKNOWN ATS/ERS Task Force on Exhaled Breath Condensate
Sep 9, 2005·Critical Care Medicine·Eric Kipnis
Nov 4, 2006·American Journal of Respiratory and Critical Care Medicine·Patryk MoskwaBotond Bánfi
Jan 6, 2007·FEBS Letters·Gregory E ConnerMatthias Salathe
Mar 22, 2007·Chemical Research in Toxicology·Michael J FascoGeorge A Eadon
Apr 25, 2007·IUBMB Life·Rita CipollonePaolo Visca
Jun 4, 2008·Folia Histochemica Et Cytobiologica·Łukasz MinarowskiElzbieta Chyczewska
Apr 13, 2010·Chest·Zsófia LázárIldikó Horváth
Jul 22, 2010·Free Radical Biology & Medicine·Eline ThomsonElizabeth Williamson
Feb 22, 2011·Free Radical Biology & Medicine·Daniel LorentzenBotond Bánfi
Mar 4, 2011·Chemical Research in Toxicology·Michael J FascoKenneth M Aldous
May 27, 2011·The Journal of Clinical Endocrinology and Metabolism·Angela M LeungElizabeth N Pearce
Apr 5, 2012·Journal of Analytical Toxicology·Stephanie L YousoBrian A Logue
Sep 13, 2012·Biochemical Pharmacology·Joshua D Chandler, Brian J Day
Apr 17, 2013·British Journal of Pharmacology·J D ChandlerB J Day
Mar 13, 2014·Respirology : Official Journal of the Asian Pacific Society of Respirology·Andras BikovIldiko Horvath
Dec 10, 2014·American Journal of Respiratory Cell and Molecular Biology·Joshua D ChandlerBrian J Day
Jan 8, 2015·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Timothy J KiddUNKNOWN ACFBAL Study Investigators
Jan 8, 2015·Free Radical Research·Joshua D Chandler, Brian J Day
May 28, 2016·The European Respiratory Journal·Anne H NeerincxPeter J F M Merkus
Apr 14, 2017·Respiratory Research·Charmion Cruickshank-QuinnNichole Reisdorph

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
Balazs AntusKrisztina Kelemen
© 2022 Meta ULC. All rights reserved