Determination of thiol metabolites in human urine by stable isotope labeling in combination with pseudo-targeted mass spectrometry analysis

Scientific Reports
Ping LiuYu-Qi Feng

Abstract

Precursor ion scan and multiple reaction monitoring scan (MRM) are two typical scan modes in mass spectrometry analysis. Here, we developed a strategy by combining stable isotope labeling (IL) with liquid chromatography-mass spectrometry (LC-MS) under double precursor ion scan (DPI) and MRM for analysis of thiols in 5 types of human cancer urine. Firstly, the IL-LC-DPI-MS method was applied for non-targeted profiling of thiols from cancer samples. Compared to traditional full scan mode, the DPI method significantly improved identification selectivity and accuracy. 103 thiol candidates were discovered in all cancers and 6 thiols were identified by their standards. It is worth noting that pantetheine, for the first time, was identified in human urine. Secondly, the IL-LC-MRM-MS method was developed for relative quantification of thiols in cancers compared to healthy controls. All the MRM transitions of light and heavy labeled thiols were acquired from urines by using DPI method. Compared to DPI method, the sensitivity of MRM improved by 2.1-11.3 folds. In addition, the concentration of homocysteine, γ-glutamylcysteine and pantetheine enhanced more than two folds in cancer patients compared to healthy controls. Taken together, the...Continue Reading

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Citations

Aug 5, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ke HeYu-Qi Feng
Sep 27, 2018·Analytica Chimica Acta·Foteini A KappiDimosthenis L Giokas

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Methods Mentioned

BETA
electrophoresis
urine samples collection

Software Mentioned

ChemSpider
AB SCIEX Analyst
QTOF
Bruker Daltonics Control
Bruker Daltonics Data analysis

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