PMID: 2490555Jan 1, 1989Paper

Determination of thiols of biological and pharmacological interest by high-performance thin-layer chromatography and fluorescence scanning densitometry

Journal of Pharmaceutical and Biomedical Analysis
B L LingK Stragier

Abstract

The application of high-performance thin-layer chromatography (HPTLC) with fluorescence scanning densitometry provides a simple, rapid and reliable system for the qualitative and quantitative determination of several thiols of biological and pharmacological interest. The determination of a mixture of thiols (captopril, coenzyme A, cysteamine, cysteine and glutathione), together with their disulphides may readily be performed by pre-chromatographic derivatization with the thiol-specific fluorobenzoxadiazole reagents SBD-F and ABD-F, followed by HPTLC separation on silica gel plates using isopropyl ether-methanol-water-acetic acid (9:8:2:1, v/v/v/v) as the developing solvent, and fluorodensitometric measurement of the fluorescing derivatives. Detection limits of about 30 pg (coenzyme A) to 6 pg (cysteamine) per spot were achieved; the relative standard deviation (RSD) of the complete procedure was 1.16-3.2%.

References

Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·W R Baeyens, B L Ling
Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·B L LingP De Moerloose
Jan 1, 1988·Journal of Pharmaceutical and Biomedical Analysis·M A MartinB del Castillo

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Citations

Sep 23, 1994·Journal of Chromatography. B, Biomedical Applications·K Shimada, K Mitamura
Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·B L LingP De Moerloose
Jan 1, 1994·Journal of Pharmaceutical and Biomedical Analysis·A M Di PietraV Cavrini
Apr 2, 2003·Journal of Pharmaceutical and Biomedical Analysis·Mehdi Bakavoli, Massoud Kaykhaii
Jun 1, 1996·Journal of Pharmaceutical and Biomedical Analysis·Z D ZhangG Van der Weken
Jul 22, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yusuke IwasakiHiroyuki Nakazawa

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