Determination of tianeptine in human plasma using high-performance liquid chromatography with fluorescence detection

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Sevgi Tatar Ulu

Abstract

A new, selective and sensitive high-performance liquid chromatography (HPLC) method with fluorimetric detection was developed for the determination of tianeptine (TIA) in human plasma using solid phase extraction (SPE) procedures. The method is based on the derivatization of TIA with 4-chloro-7-nitrobenzofurazan (NBD-Cl) in borate buffer of pH 8.5 to yield a yellow, fluorescent product. The HPLC separation was achieved on a Phenomenex C(18) column (250 mm x 4.6 mm) using a mobile phase of acetonitrile-10mM orthophosphoric acid (pH 2.5) (77:23, v/v) solvent system at 1 mL/min flow rate. Gabapentin (GA) was used as the internal standard. The fluorometric detector was operated at 458 nm (excitation) and 520 nm (emission). The assay was linear over the concentration range of 5-300 ng/mL. The detection limit (LOD) was found to be 2 ng/mL. The mean recovery was determined to be 88.6%. The proposed method was applied for pharmacokinetic study of 12.5mg TIA in a healthy volunteer.

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Citations

Dec 24, 2015·Talanta·Ramzia I El-BagaryMarwa A El-Wahab Mohamed
Jul 17, 2007·Forensic Science International : Synergy·Paula ProençaDuarte Nuno Vieira
Jun 27, 2012·Journal of Separation Science·Juan José Fernández-NavarroMaría Celia García-Álvarez-Coque
Dec 7, 2007·Chemical & Pharmaceutical Bulletin·Nahed El-EnanyFathalla Belal
Dec 2, 2008·Chemical & Pharmaceutical Bulletin·Sevgi Tatar Ulu, Zeynep Aydogmus
Feb 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xinkun ShenKaiyong Cai

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