Determination of tobacco-specific N-nitrosamines in rabbit serum by capillary zone electrophoresis and capillary electrophoresis-electrospray ionization-mass spectrometry with solid-phase extraction

Electrophoresis
Chenchen LiHuwei Liu

Abstract

In this paper, we propose a new strategy for separation and determination of tobacco-specific N-nitrosamines (TSNAs), a group of strong carcinogens found only in tobacco products, by using CZE and CE-MS associated with SPE. Six TSNAs: N'-nitrosonornicotine, N'-nitrosoanatabine, N'-nitrosoanabasine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, and 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanol were simultaneously separated by either of two CZE methods, one of which worked with ammonium formate buffer (pH 2.5) and another with citrate buffer (pH 2.4), as well as a CE-MS method. The CZE conditions including pH and concentration of running buffer, capillary length, applied voltage, and capillary temperature were systematically optimized. For CE-MS method, an optimized sheath liquid consisted of methanol-water was used at a flow rate of 10 muL/min. With SPE procedure, our proposed CE-MS method was successfully applied to determine TSNAs after 15 min metabolism in rabbits. A comparison study between CZE and CE-MS methods for quantitative purposes was carried out, showing that both methods provided similar separation efficiency, selectivity, repeatability, linearity, and recovery. Howev...Continue Reading

References

Jun 24, 1998·Chemical Research in Toxicology·S S Hecht
Mar 5, 1999·Mutation Research·S S Hecht
Jul 27, 2004·Electrophoresis·Javier Hernández-BorgesMatthias Pelzing
Mar 12, 2005·Electrophoresis·Carolina SimóAlejandro Cifuentes
Mar 15, 2005·Electrophoresis·David C Simpson, Richard D Smith
Mar 19, 2005·Electrophoresis·Philippe Schmitt-Kopplin, Matthias Englmann

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Citations

Nov 14, 2006·Journal of Chromatography. a·Javier Hernández-BorgesAlejandro Cifuentes
Feb 28, 2007·Journal of Mass Spectrometry : JMS
Dec 29, 2007·Electrophoresis·Andras GasparPhilippe Schmitt-Kopplin

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