Determination of tobramycin in human serum by capillary electrophoresis with contactless conductivity detection

Electrophoresis
Wai Siang LawPeter C Hauser

Abstract

A study on the determination of the antibiotic tobramycin by CE with capacitively coupled contactless conductivity detection is presented. This method enabled the direct quantification of the non-UV-absorbing species without incurring the disadvantages of the indirect approaches which would be needed for optical detection. The separation of tobramycin from inorganic cations present in serum samples was achieved by optimizing the composition of the acetic acid buffer. Field-amplified sample stacking was employed to enhance the sensitivity of the method and a detection limit of 50 microg/L (S/N = 3) was reached. The RSDs obtained for migration time and peak area using kanamycin B as internal standard were typically 0.12 and 4%, respectively. The newly developed method was validated by measuring the concentration of tobramycin in serum standards containing typical therapeutic concentrations of 2 and 10 mg/L. The recoveries were 96 and 97% for the two concentrations, respectively.

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Citations

May 4, 2010·Analytical and Bioanalytical Chemistry·Leena Suntornsuk
Sep 25, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Thi Thanh Thuy PhamPeter C Hauser
Oct 22, 2011·Journal of Pharmaceutical and Biomedical Analysis·Mohamed Nouri El-AttugAnn Van Schepdael
Mar 26, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Worapan PormsilaPeter C Hauser
Nov 6, 2010·Biosensors & Bioelectronics·Eva González-FernándezPaulino Tuñón-Blanco
Jan 2, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chang-Zhu YuWu-Er Gan
Nov 24, 2007·Journal of Chromatography. a·Steven L SimpsonShigeru Terabe
Dec 23, 2008·Electrophoresis·Zdena MaláPetr Bocek
Dec 12, 2007·Electrophoresis·Elaine Teng Teng TaySam Fong Yau Li
Nov 28, 2007·Electrophoresis·María Castro-PuyanaMaría L Marina
Dec 25, 2007·Analytica Chimica Acta·Pavel Kubán, Peter C Hauser

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