Determination of tocopherol and tocopherol acetate concentrations in human feces using high-performance liquid chromatography

Journal of Chromatography
D W NierenbergT A Colacchio

Abstract

A method of sample clarification and high-performance liquid chromatography (HPLC) specifically developed to permit simple and rapid determination of vitamin E (alpha-tocopherol, E) and vitamin E acetate (EA) in feces is reported. Retinol acetate (RA) was used as an internal standard. The vitamins of interest were extracted from an aqueous stool suspension into an organic phase (ethyl acetate-butanol), which was injected directly onto the reversed-phase HPLC system. An isocratic mobile phase of methanol-water (97:3) was employed, with ultraviolet detection at 275 and 285 nm (to permit simultaneous monitoring and absorbance ratio determination). Recoveries of exogenous RA, E, and EA from stool suspensions (relative to water) were 99.0 +/- 7.0, 100.9 +/- 7.0, and 101.2 +/- 13.3%, respectively (n = 10). The organic matrix could be stored at -35 degrees C overnight with no change in E or EA results. Sensitivities for E and EA were 80 and 102 micrograms/g of stool, respectively. Each analysis required nine min. The within-day coefficients of variation were 2.9, 3.6, and 3.0% (n = 7) for RA, E, and EA, respectively. Neither E nor EA were detected in baseline fecal samples from fourteen subjects, but both were present in high but vari...Continue Reading

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Citations

Feb 20, 2010·Molecular Nutrition & Food Research·Jean-Marc ZinggAngelo Azzi
Jan 11, 2005·The American Journal of Clinical Nutrition·Barry HalliwellAndrew Jenner
Apr 29, 1998·Journal of the American College of Nutrition·C C TangneyW Piccione
Jul 29, 1988·Journal of Chromatography·A P De LeenheerR M Bauwens
Apr 28, 2004·Biochemical and Biophysical Research Communications·Adelina MunteanuAngelo Azzi
Jul 12, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Stéphane CastanEl Hassan Ajandouz
May 20, 2008·Chemistry and Physics of Lipids·Lydie BrissonEl Hassan Ajandouz

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