Determination of tocopherols and their metabolites by liquid-chromatography coupled with tandem mass spectrometry in human plasma and serum

Talanta
Danilo GiusepponiRoberta Galarini

Abstract

Several studies are increasingly underlying the biological role of vitamin E metabolites as bioactive compounds with anti-inflammatory, anti-proliferative and anti-atherogenic activity. A quantitative method for the simultaneous determination in human plasma and serum of vitamin E (α-tocopherol, α-T and γ-tocopherol, γ-T) and its cytochrome P-450 metabolites: 13'-hydroxychromanol (α-13'-OH), 13'-carboxychromanol (α-13'-COOH) and carboxyethyl hydroxychromanols (α-CEHC and γ-CEHC), was developed and validated. After enzymatic hydrolysis and deproteinization, the metabolites were extracted with a mixture of hexane/ methyl tertiary butyl ether (2/1, v/v). The separation was achieved by reversed phase chromatography and the analytes detected by a triple quadrupole mass analyser using electrospray ionization in positive mode (LC-MS/MS). α-T and γ-T were extracted separately without enzymatic hydrolysis. The analytes were quantified with the isotopic dilution method. After an extensive validation study (three levels in three different occasions for a total of 54 experiments), the procedure was successfully applied to the analysis of sera of healthy volunteers (before and after supplementation with α-T) and plasma of patients affected ...Continue Reading

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Citations

Mar 27, 2018·Rapid Communications in Mass Spectrometry : RCM·E BartosińskaP Wiczling
Dec 15, 2018·IUBMB Life·Fatemeh Khadangi, Angelo Azzi
Dec 24, 2018·IUBMB Life·Marc Birringer, Stefan Lorkowski
Sep 22, 2018·Nature Communications·Helmut PeinAndreas Koeberle
Feb 19, 2019·Oxidative Medicine and Cellular Longevity·Vassilios LiakopoulosEvangelia Dounousi
Apr 14, 2021·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Hadi HabibBenjamin C Moeller

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