Determination of tolterodine and the 5-hydroxymethyl metabolite in plasma, serum and urine using gas chromatography-mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis
L PalmérU Stenberg

Abstract

A specific and sensitive capillary gas chromatography-mass spectrometry assay for the determination of tolterodine and the 5-hydroxymethyl metabolite (Labcode DD 01) in plasma, serum and urine is described. Extraction of the analytes was performed with liquid/liquid or solid-phase extraction prior to derivatisation with a silyl reagent. The derivatives were quantified by selected ion monitoring mass spectrometry using deuterium-labelled internal standards. A single level calibration curve was utilised for quantification of plasma, serum and urine concentrations of tolterodine and DD 01. The accuracy (inter- and intra-day) for both analytes was within 87-110% in the range 0.5 and 50 ng ml-1 and precision was better than 90%. Overall, this method was shown to be reliable for pharmacokinetic assays of tolterodine and the metabolite DD 01 in samples from preclinical and clinical studies.

Citations

Apr 12, 2001·BJU International·K HjälmåsA Stenberg
Mar 28, 2001·Journal of Clinical Pharmacology·B OlssonH Arnberg
Nov 11, 2003·Clinical Pharmacokinetics·David R P Guay
Apr 17, 1999·Biopharmaceutics & Drug Disposition·I Påhlman, P Gozzi
Jun 19, 1998·Clinical Pharmacology and Therapeutics·N BrynneJ Gabrielsson
Dec 3, 1999·British Journal of Clinical Pharmacology·N BrynneL Bertilsson
Dec 3, 1999·British Journal of Clinical Pharmacology·N BrynneL Bertilsson

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