Determination of total antioxidant capacity of milk by CUPRAC and ABTS methods with separate characterisation of milk protein fractions

The Journal of Dairy Research
Sema Demirci CekiçReşat Apak

Abstract

Most milk-applied antioxidant assays in literature are based on the isolation and quantification of individual antioxidative compounds, whereas total antioxidant capacity (TAC) gives a more holistic picture due to cooperative action of antioxidants. Recently, the cupric reducing antioxidant capacity (CUPRAC) method has been modified to measure the antioxidant capacities of thiol-containing proteins, where the classical ammonium acetate buffer - that may otherwise precipitate proteins- was replaced with concentrated urea buffer (able to expose embedded thiol groups of proteins to oxidative attack) adjusted to pH 7.0. Thus, antioxidant capacity of milk was investigated with two competing TAC assays, namely CUPRAC and ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid))/persulphate, because only these assays were capable of evaluating protein contribution to the observed TAC value. As milk fat caused turbidity, experiments were carried out with skim milk or defatted milk samples. To determine TAC, modified CUPRAC method was applied to whole milk, separated and redissolved protein fractions, and the remaining liquid phase after necessary operations. Both TAC methods were investigated for their dilution sensitivity and anti...Continue Reading

References

Jun 25, 1999·Free Radical Biology & Medicine·R ReC Rice-Evans
Apr 1, 2000·The Journal of Nutritional Biochemistry·K SuetsunaH Ochi
Apr 12, 2000·Biochemistry·W J WedemeyerH A Scheraga
Jan 12, 2001·Analytical Biochemistry·I O Egwim, H J Gruber
Sep 10, 2003·The Journal of Biological Chemistry·Masanori YagiYuji Goto
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Apr 15, 2005·Journal of Dairy Science·N SavaM Hendrickx
Mar 31, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eszter PappPéter Csermely
May 9, 2008·Critical Reviews in Food Science and Nutrition·Ryan J EliasEric A Decker
Nov 1, 2009·Analytical Methods : Advancing Methods and Applications·Tomer NoyhouzerDaniel Mandler

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Citations

Aug 25, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Kunio YuiYohei Kawasaki

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