Determination of total mercury and methylmercury in human hair by graphite-furnace atomic absorption spectrophotometry using 2,3-dimercaptopropane-1-sulfonate as a complexing agent

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Wen-Yi ChenMao-Sung Kuo

Abstract

For the determination of total mercury in hair, an amount (25.0 mg) of hair sample was digested with conc. HNO3 (400 microl) at 90 degrees C for 10 min in a 7-ml teflon microreaction vessel. After digestion, the pH of the acidic hair mixture was adjusted to 5.0-6.0 by NaOH and was then passed through a clean-up Sep-Pak C18 cartridge. To the eluate, 2,3-dimercaptopropane-1-sulfonate (DMPS) and sodium acetate buffer (pH = 6.0) were added to form a mercury-DMPS complex. This complex was preconcentrated on two Sep-Pak C18 cartridges in series, and each cartridge was eluted with methanol and adjusted to 2.00 ml. A portion (50 microl) was introduced into a graphite cuvette and then atomized according to a temperature program. The method detection limit (MDL, 3sigma) was 0.064 (microg g(-1)); the calibration graph was linear up to 7.52 microg g(-1). Good accuracies were obtained when testing two human hair certified reference materials (GBW 09101 and BCR-397). Six real samples were analyzed, and the recoveries were 95.8 - 98.2% with a relative standard deviation (RSD, n = 3) < 2.1%. For the determination of methylmercury (CH3Hg+), 25.0 mg of hair sample was extracted with 2.0 mol dm(-3) HCl (1.0 ml) by ultrasonicating for 1 h. The sup...Continue Reading

References

Jan 1, 1995·Chemosphere·R Nakagawa
Dec 20, 1996·The Science of the Total Environment·J BatistaJ Corbella
Mar 26, 1998·The Science of the Total Environment·S C Foo, T C Tan
Oct 1, 1998·Environmental Toxicology and Pharmacology·A C Barbosa, J G Dórea

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Citations

Apr 10, 2010·Applied Microbiology and Biotechnology·He WeiLin Xian-Gui
Aug 29, 2013·Environmental Science & Technology·Xueqing XingZhonghua Wu
Mar 21, 2007·Environmental Health Perspectives·Fei XueLawrence Fischer
May 31, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Chia-Chen Li, Mao-Sung Kuo
Jun 13, 2006·The Science of the Total Environment·Paolo MontuoriJosep M Bayona
Dec 28, 2019·Journal of Wildlife Diseases·Jill C WittJoseph Bump

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