PMID: 9530609Apr 8, 1998Paper

Determination of transforming growth factor-beta 2 (TGF-beta 2) in bovine colostrum samples

Journal of Immunoassay
R Pakkanen

Abstract

Transforming growth factor-beta 2 (TGF-beta 2) is the major TGF-beta form in bovine colostrum. A colostrum pool of the five first milkings was made to validate an ELISA specific for human TGF-beta 2 for measure TGF-beta 2 concentration in bovine colostrum samples. According to this test > 90% of total TGF-beta 2 (74.5 +/- 4.4 ng/ml) in colostrum pool was in a latent form that could be activated by acetic acid treatment, whereas the concentration of the active form was only 4.19 +/- 0.27 ng/ml. Activated colostrum samples of the first milkings of five cows contained 150-1150 ng TGF-beta 2/ml and its concentration declined in correlation (r = 0.86) with total protein concentration to 12-71 ng/ml by the fifth milkings. Most of the TGF-beta 2 (94%) was found in the whey fraction of colostrum. The ELISA results were also compared with a TGF-beta 2 bioassay, the fibroblasts migration assay. This assay detected 9.8 +/- 1.0 ng/ml and 4.4 +/- 0.7 ng/ml in the activated and non-activated samples of colostrum pool respectively.

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Citations

Apr 23, 2009·Reproduction, Fertility, and Development·Leanne J McGrathSarah A Robertson
Aug 27, 2003·The Journal of Veterinary Medical Science·Hitoki YamanakaHiroshi Iwai
Nov 16, 2004·Biology of the Neonate·Kirsi LaitinenErika Isolauri
Dec 23, 2016·Microbial Pathogenesis·Eduardo Sánchez-SotoSara E Herrera-Rodríguez
Jan 23, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·P T SangildR J Xu
Apr 6, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kopperuncholan NamachivayamAkhil Maheshwari
Jul 18, 2017·Journal of Exercise Nutrition & Biochemistry·Tae-Woon KimJinhee Seo
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kinga Kazimierska, Urszula Kalinowska-Lis
Jun 23, 2005·Veterinary Immunology and Immunopathology·Douglas D BannermanJayne C Hope

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