Determination of tributyrin and its metabolite butyrate in Wistar rat plasma samples by gas chromatography/mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Jie SuPaul C Ho

Abstract

A gas chromatographic (GC) method with mass spectrometric (MS) detection was developed for the determination of tributyrin and its metabolite butyrate in rat plasma. Following precipitation of plasma protein with acetonitrile, the analytes in the samples were separated on a DB-5ms capillary column with helium as carrier gas. Phenylmethylsulfonyl fluoride (PMSF), an inhibitor for serine proteases, papain and acetylcholinesterase, was found to be essential to inhibit the activity of enzyme(s) responsible for the hydrolysis of tributyrin in both rat and human blood samples. The enzyme inhibitor in 5 mM (final concentration) was added immediately into the blood samples after collection to prevent the hydrolysis. The linear concentration ranges for tributyrin and butyrate were 0.1-2.0 and 1-20 microM, respectively. The coefficients of variation for intra-day and inter-day assays for tributyrin were all <10%, and those for butyrate were also <10%, except for the lowest concentration (1 microM), which was less than 20%. The accuracy of all concentration determinations ranged from 96.0-110.0%. The limit of quantification (LOQ) was 0.1 microM for tributyrin and 1.0 microM for butyrate. This method could detect tributyrin and butyrate si...Continue Reading

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Citations

Feb 6, 2009·International Journal of Cancer. Journal International Du Cancer·Joice Kuroiwa-TrzmielinaFernando Salvador Moreno
Dec 5, 2013·International Journal of Cancer. Journal International Du Cancer·Aline H GuarientoFernando S Moreno
May 25, 2012·American Journal of Physiology. Endocrinology and Metabolism·Marco Aurélio Ramirez VinoloRui Curi
Mar 19, 2020·Parasitology Research·Wenling HuangHuifen Dong

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