Determination of trichothecene mycotoxins in wheat by use of supercritical fluid extraction and high-performance liquid chromatography with diode array detection or gas chromatography with electron capture detection

Journal of Chromatography. a
R D JosephsJ A Broekaert

Abstract

The extraction behaviour of the Fusarium mycotoxin deoxynivalenol (DON) and some related type B trichothecenes from spiked seasand, spiked wheat flour and naturally contaminated wheat flour with modified supercritical CO2 has been investigated and optimized under several conditions. The extraction fluid was decompressed over a solid-phase trap and the amount of deposited analytes was determined by HPLC-diode array detection (DAD) or GC-electron capture detection (ECD) without any further clean-up. Recovery rates as high as 90.1 +/- 10.7% were achieved for spiked wheat samples and 53.0 +/- 3.2% for naturally contaminated samples. The performance of the optimized supercritical fluid extraction (SFE) method was compared with an already well established analytical method employing extraction on a rotary shaker in combination with Mycosep clean-up. Moreover, the SFE procedure developed for naturally DON contaminated wheat was employed for the simultaneous extraction of 5 type B trichothecenes by GC-ECD. This work represents the first successful approach in obtaining an SFE-method for the extraction of Fusarium mycotoxins from wheat with reasonable recoveries and good precision.

Citations

Mar 27, 2010·Applied Microbiology and Biotechnology·Robert KöppenIrene Nehls
Oct 14, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·E Razzazi-FazeliJ Zentek
Dec 27, 2008·Analytica Chimica Acta·Nicholas W TurnerSergey A Piletsky
Nov 6, 2013·Critical Reviews in Food Science and Nutrition·Jinyi YangDharini Sivakumar
Sep 18, 2003·Journal of Agricultural and Food Chemistry·Anna J TüdösEdwin C A Stigter

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