Determination of trimethoprim metabolites including conjugates in urine using high-performance liquid chromatography with combined ultraviolet and electrochemical detection

Journal of Chromatography
L Nordholm, L Dalgaard


A high-performance liquid chromatographic method for the determination of trimethoprim metabolites in pig urine was developed. The metabolites-glucuronic acid and sulphuric acid conjugates of phenolic metabolites formed by demethylation of trimethoprim-were quantitated after treatment of urine with beta-glucuronidase (Escherichia coli). The sulphuric acid conjugate was not susceptible to enzymatic hydrolysis and was therefore assayed as the conjugate by use of ion-pair chromatography on the reversed-phase column. In order to find suitable conditions for enzymatic hydrolysis of the glucuronides, the conjugates were obtained by gel chromatography of urine from a [14C] trimethoprim-treated pig.


Nov 1, 1979·Antimicrobial Agents and Chemotherapy·D M LichtenwalnerA M Sugar
Sep 11, 1979·Journal of Chromatography·R E Weinfeld, T C Macasieb
Jul 1, 1978·Journal of Chromatography·G LandA Bye
Nov 1, 1978·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·P Nielsen, L Dalgaard
Sep 1, 1977·Journal of Chromatographic Science·A Bye, M E Brown
Nov 1, 1973·The Journal of Infectious Diseases·C W SigelC A Nichol
Aug 1, 1974·Journal of Pharmaceutical Sciences·C W SigelG H Hitchings

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Feb 27, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·K Hoppu, P Arjomaa
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