Determination of UCP1 expression in subcutaneous and perirenal adipose tissues of patients with hypertension

Endocrine
Xueqin LiGuoxian Ding

Abstract

The objective of this study is to determine the property of human perirenal adipose tissue (PAT) and assess the adipose property of PAT in hypertension. Ninety-four patients, including 64 normotensive patients (T-NP) and 30 hypertensive patients (HP), who underwent renal surgery were included. Expression analysis was performed using quantitative real-time polymerase chain reaction, Western blot, and immunohistochemistry in PAT and back subcutaneous adipose tissue (bSAT) depots. Compared with bSAT, PAT adipocytes were smaller, and the expressions of uncoupling protein-1 (UCP1) mRNA and protein were markedly higher, while the mRNA expressions of markers for classic beige and white adipocytes were lower in PAT. Immunohistochemistry analysis showed more multilocular UCP1-positive adipocytes in PAT than in bSAT. UCP1 expressions were lower in PAT in HP than in the T-NP or age- and body mass index-matched NP groups. Bigger unilocular adipocytes with less UCP1 staining in PAT were detected in HP than in NP group, although no such difference was observed in bSAT. PAT acts as a brown-like fat. UCP1 expression of PAT was lower in HP than in normotensive patients. UCP1 expression of PAT may serve as a protective indicator for hypertension.

References

Apr 1, 1995·Hypertension·K T StepniakowskiB M Egan
Feb 8, 2003·Science·Jeffrey M Friedman
May 16, 2003·JAMA : the Journal of the American Medical Association·Aram V ChobanianUNKNOWN National High Blood Pressure Education Program Coordinating Committee
Aug 12, 2003·Nature Genetics·Zhihong ZhouPeng Li
Jan 17, 2004·The Biochemical Journal·Stephen J Yeaman
May 10, 2005·The Proceedings of the Nutrition Society·Daniel Ricquier
Aug 23, 2008·Nature·Patrick SealeBruce M Spiegelman
Mar 30, 2010·The Journal of Biological Chemistry·John E HallDavid E Stec
Jul 28, 2010·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·A Gastaldelli, G Basta
Jan 22, 2011·Nature Reviews. Immunology·Noriyuki OuchiKenneth Walsh
Jul 9, 2011·Journal of Atherosclerosis and Thrombosis·Yuji MatsuzawaTadashi Nakamura
Sep 14, 2011·Clinical Science·Theodora Szasz, R Clinton Webb
Sep 21, 2011·Hypertension·Meredith C FosterCaroline S Fox
Oct 11, 2011·Menopause : the Journal of the North American Menopause Society·Tiziana MontalciniArturo Pujia
Mar 8, 2012·Nature Medicine·Olivia Osborn, Jerrold M Olefsky
Jun 6, 2012·Circulation·Charmaine S TamEric Ravussin
Dec 12, 2012·The Journal of Clinical Investigation·Kristin I StanfordLaurie J Goodyear
Apr 4, 2013·Endocrine Reviews·Paul LeeKen K Y Ho
Apr 23, 2013·Nature Medicine·Martin E LidellSven Enerbäck
Apr 30, 2013·Nature Cell Biology·Matthias RosenwaldChristian Wolfrum
Jul 23, 2013·Metabolism: Clinical and Experimental·Johan Van de VoordeKelly Decaluwé
Dec 19, 2013·International Journal of Endocrinology·Gianluca IacobellisClaudio Letizia
Apr 23, 2014·Obesity·Per-Arne SvenssonMaria K Svensson
Oct 15, 2014·The Journal of Clinical Endocrinology and Metabolism·Claudio LetiziaGianluca Iacobellis

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Citations

Jul 28, 2015·Metabolism: Clinical and Experimental·Mohammed K HankirWiebke K Fenske
Jun 10, 2016·Journal of Cellular Physiology·Nevena JeremicSuresh C Tyagi
Jul 21, 2016·American Journal of Physiology. Endocrinology and Metabolism·Yuan ZhangLi-Jun Yang
Apr 17, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Robrecht ThoonenMarielle Scherrer-Crosbie
Jun 17, 2020·The Biochemical Journal·Alice E Pollard, David Carling
Jan 23, 2020·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Jisun LimYoonseok Heo
Dec 8, 2020·Frontiers in Cardiovascular Medicine·Ibrahim AlZaimAhmed F El-Yazbi
Aug 20, 2021·Frontiers in Endocrinology·Safaa H HammoudAhmed F El-Yazbi

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