PMID: 3890726Apr 1, 1985Paper

Determination of vancomycin in human serum by high-pressure liquid chromatography

Antimicrobial Agents and Chemotherapy
F JehlH Monteil

Abstract

A rapid, accurate, reverse-phase high-pressure liquid chromatographic procedure for vancomycin quantitation in human serum, cerebrospinal fluid, and peritoneal fluid was developed. This procedure involves a simple chemical extraction of the antibiotic and is suitable for each of these body fluids. The column and mobile phase used provided a good resolution of the vancomycin peak with a retention time of 6.1 min. The precision of the assay was within the requirement for a daily routine clinical application. Coefficients of variation for within-day reproducibility were 5.80 and 6.28%, respectively, for samples at 50 and 25 micrograms/ml, and for between-day reproducibility they were 11.4 and 11.1%, respectively. No interference was found with respect to beta-lactam and aminoglycoside antibiotics and many other currently used drugs, indicating a good specificity for the procedure. The detection limit of 100 ng/ml has proven to be sufficient for monitoring drug levels in serum obtained after usual dosages. Drug levels in 112 clinical serum specimens assayed by high-pressure liquid chromatography were regressed against the levels obtained for the same samples by radioimmunoassay and fluorescent polarization immunoassay. Correlation ...Continue Reading

References

Jun 1, 1978·Annals of Internal Medicine·F V Cook, W E Farrar
Jan 1, 1979·Therapeutic Drug Monitoring·J R Uhl, J P Anhalt
Mar 1, 1981·Annals of Internal Medicine·Robert C MoelleringD J Greenblatt
Jan 1, 1983·Antimicrobial Agents and Chemotherapy·B F Farber, Robert C Moellering
Jan 1, 1982·Pharmacology & Therapeutics·H R Perkins
Feb 1, 1983·The Journal of Infectious Diseases·C W Norden, M Shaffer
Apr 1, 1983·Antimicrobial Agents and Chemotherapy·N S BrownG P Bodey
Jan 1, 1982·The Journal of Antimicrobial Chemotherapy·M T CafferkeyC T Keane
Sep 10, 1982·Journal of Chromatography·J B McClainS Brown
Jan 1, 1981·Antimicrobial Agents and Chemotherapy·K L FongG P Bodey
Apr 1, 1981·Antimicrobial Agents and Chemotherapy·R M RatcliffR White
Dec 1, 1981·Antimicrobial Agents and Chemotherapy·M S EngineerG P Bodey
Nov 1, 1981·Reviews of Infectious Diseases·B A CunhaC H Nightingale
Nov 1, 1981·Reviews of Infectious Diseases·R FeketyR Toshniwal
Apr 1, 1980·Journal of Clinical Pharmacology·D J KrogstadD J Greenblatt

Citations

Apr 1, 1987·Antimicrobial Agents and Chemotherapy·R N GreenbergR McMillian
May 1, 1993·Antimicrobial Agents and Chemotherapy·H SunA R Krusell
Jul 1, 1990·Journal of Pharmaceutical Sciences·L S WangJ D Huang
Jul 22, 2008·Journal of Pharmaceutical and Biomedical Analysis·María José de Jesús ValleAmparo Sánchez Navarro
Nov 21, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·K J Vera LópezS R C Jorge Santos
Jun 22, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Pang-Hsin HsiehChun-Hsiung Shih
Feb 1, 2006·Rapid Communications in Mass Spectrometry : RCM·J DianaE Adams
Mar 12, 2010·International Journal of Antimicrobial Agents·Daria NicolosiRosario Pignatello
Oct 27, 2017·Journal of Chromatographic Science·Tácio de Mendonça LimaRita de Cássia Elias Estrela
Feb 18, 1998·Clinical Pharmacology and Therapeutics·F Schaedeli, D E Uehlinger
Feb 20, 1996·Biotechnology and Bioengineering·J J McIntyreA W Bunch
Jul 1, 2020·Biomaterials·Emma WatsonAntonios G Mikos
Oct 12, 1990·Journal of Chromatography·F JehlH Monteil
May 19, 1995·Journal of Chromatography. B, Biomedical Applications·J Luksa, A Marusic

Related Concepts

Bacterial Infections
High Pressure Liquid Chromatography Procedure
Fluorescence Polarization
Immunofluorescence Assay
Radioimmunoassay
AB-Vancomycin

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