Abstract
Background:The gas human exhaled contains many volatile organic compounds (VOCs), which is related to the health status of body. Analysis of VOCs has been proposed as a noninvasive diagnostic tool for certain cancers. Detailed research on the VOCs in gas exhaled by cell can characterize cell type specific metabolites and may be helpful to detect the cancer markers in clinical practice.Methods:Solid-phase microextraction (SPME) gas chromatography-mass spectrometry was used to detect VOCs in the headspace of tissue culture flask in non-Hodgkin's lymphoma (NHL) cell line JEKO and acute mononuclear leukemia cell line SHI-1, to elaborate the characteristic gaseous biomarkers of hematological malignancies. While macrophage cells and lymphocytic cells were acted as control. The blank group was only the RPMI 1640 medium containing 10% fetal calf serum that without cells.Results:Comparing with control group, the concentration of dimethyl sulfide, 2,4-dimethylheptane, methylbenzene,o-xylene, dodecane, and 1,3-di-tert-butylbenzene in JEKO cells was relatively higher, while the concentration of ethanol, hexanal, and benzaldehyde was lower. In SHI-1 cells, the levels of 2,4-dimethylheptane, benzene, 4-methyldecane, chloroform, 3,7-dimethyl ...Continue Reading
References
Jul 1, 1992·Scientific American·M Phillips
Jun 11, 2003·Chest·Michael PhillipsWilliam N Rom
Feb 12, 2005·Respiratory Physiology & Neurobiology·Berthold MoserPhilipp Lirk
May 25, 2005·Indoor Air·H SchleibingerH Ruden
Jul 16, 2005·Respiratory Research·Diana PoliAntonio Mutti
Dec 31, 2005·Blood·Ann ZeunerRuggero De Maria
Nov 3, 2006·Angewandte Chemie·Huanwen ChenRenato Zenobi
Jan 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Emel Calişkan-CanSevgi Yardim-Akaydin
Mar 20, 2008·Rapid Communications in Mass Spectrometry : RCM·Ruyi XueXizhong Shen
Apr 17, 2008·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·A D'AmicoC Di Natale
Jun 25, 2008·Mutation Research·Parag P ShahDevendra Parmar
Oct 28, 2008·Chemico-biological Interactions·Deniz UcarJan S Moreb
Nov 21, 2008·Blood·Asa Rangert DerolfMagnus Björkholm
Jan 9, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Wojciech FilipiakJakob Troppmair
Jul 22, 2010·British Journal of Cancer·G PengH Haick
Sep 9, 2010·Industrial Health·Jin Hee KimYun-Chul Hong
Jan 26, 2011·Cancer Biomarkers : Section a of Disease Markers·Andreas SponringJakob Troppmair
Oct 29, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Orna BarashHossam Haick
Dec 14, 2011·Hematology·Gail J Roboz
Apr 10, 2012·Bioscience, Biotechnology, and Biochemistry·Yosuke HanaiHiroaki Oka
Jun 13, 2012·Cancer Prevention Research·Mohamed HassaneinPierre P Massion
Sep 11, 2012·Malaria Journal·Rina P M WongTimothy M E Davis
Nov 13, 2012·Cancer Biomarkers : Section a of Disease Markers·Yishan WangKejing Ying
Mar 7, 2013·British Journal of Cancer·Z-q XuH Haick
Mar 22, 2013·Analytical and Bioanalytical Chemistry·Qin ZhaoYu-Qi Feng
Jul 23, 2013·Cancer Cell International·Paweł MochalskiAnton Amann
Oct 9, 2013·Cancer Biology & Therapy·Changsong WangEnyou Li
May 14, 2014·Analytical and Bioanalytical Chemistry·Changsong WangEnyou Li
May 28, 2014·Journal of Breath Research·Wojciech FilipiakAnton Amann
Feb 7, 2015·Cancer Treatment and Research·Brian C-H Chiu, Ningqi Hou
Apr 26, 2015·Analytica Chimica Acta·Ezel BoyacıJanusz Pawliszyn
Aug 8, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Muhammad SajidHian Kee Lee
Mar 20, 2016·Journal of Chromatographic Science·Min HeHai Wu
Jul 9, 2016·PloS One·Joana A DiasElisabet Wirfält
Citations
Feb 22, 2018·Journal of Breath Research·Dibyendu DuttaSeah H Lim
Jul 13, 2019·Cells·Ann-Christin KlemenzKirsten Peters
Jul 31, 2020·Journal of Breath Research·Liam David LittleSarah Louise Haywood-Small
Sep 26, 2020·Scientific Reports·Hsiao-Chi ChuangHsiao-Yu Yang
Apr 5, 2018·Scientific Reports·Ana Rita LimaPaula Guedes de Pinho
Jan 24, 2019·Frontiers in Microbiology·Kevin HidalgoErwan Engel
Oct 8, 2020·Frontiers in Chemistry·Xiaofeng ChenZheng Li
Nov 30, 2020·Analytica Chimica Acta·Mohammad Mahdi BordbarBahram Hemmateenejad