Determination of Voriconazole Concentrations in Serum by GC-MS

Journal of Clinical Laboratory Analysis
Alisha Smith, Van Leung-Pineda

Abstract

Voriconazole is a broad spectrum triazole antifungal drug used to treat systemic fungal infections. Therapeutic drug monitoring of voriconazole is necessary for achieving maximal efficiency without inducing toxic side effects. Other publications have reported methods for measuring voriconazole in serum using high-performance liquid chromatography (HPLC) and liquid chromatography tandem mass spectrometry (LC-MS/MS). Here, we report for the first time a method for the measurement of voriconazole in serum samples using gas chromatography mass spectrometry (GC-MS). Protein precipitation with methanol was used to extract the antifungal that was derivatized with BSTFA (Sigma-Aldrich, St. Louis, MO) and analyzed by GC-MS. Linearity, sensitivity, precision, accuracy, and drug interferences were evaluated for this assay. Our method was linear up to 10 μg/ml of voriconazole. The LOQ was determined to be 0.4 μg/ml. CV for between-day precision was <12%. Correlation with an established LC-MS/MS yielded a R2 of 0.96. Tested drugs did not result in >10% error in measurement. To our knowledge, we report here the first GC-MS method for voriconazole measurement with acceptable performance. We hope that this method allows clinical laboratories w...Continue Reading

References

Sep 12, 2000·The Annals of Pharmacotherapy·J A Sabo, S M Abdel-Rahman
Sep 29, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R AllyUNKNOWN Esophageal Candidiasis Study Group
Jun 25, 2003·Antimicrobial Agents and Chemotherapy·Gennethel J PennickMichael G Rinaldi
Nov 8, 2006·Antimicrobial Agents and Chemotherapy·Andres PascualOscar Marchetti
Oct 13, 2007·Clinical Biochemistry·Loralie J Langman, Felix Boakye-Agyeman
Jan 1, 1988·The Journal of Automatic Chemistry·R Haeckel
Oct 15, 2010·Bioscience, Biotechnology, and Biochemistry·Xin XiongXi Lan
Oct 15, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kerstin Yvonne BesteVolkhard Kaever
Jul 4, 2012·Antimicrobial Agents and Chemotherapy·Michael J DoltonAndrew J McLachlan

❮ Previous
Next ❯

Citations

Mar 18, 2020·Pharmaceutical Development and Technology·Rui ChenXiaohong Xu
May 7, 2021·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Sahar YousefianFarzad Kobarfard

❮ Previous
Next ❯

Related Concepts

Related Feeds

Central Nervous System Fungal Infections

Central nervous system fungal infections are rare and typically occur in immunocompromised patients, such as those with HIV infection or transplant recipients. Discover the latest research on central nervous system fungal infections here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.