Determining antioxidant activities of lactobacilli cell-free supernatants by cellular antioxidant assay: a comparison with traditional methods

PloS One
Jiali XingWei Chen

Abstract

Antioxidant activity of lactic acid bacteria is associated with multiple health-protective effects. Traditional indexes of chemical antioxidant activities poorly reflect the antioxidant effects of these bacteria in vivo. Cellular antioxidant activity (CAA) assay was used in this study to determine the antioxidant activity of cell-free supernatants (CFSs) of 10 Lactobacillus strains. The performance of the CAA assay was compared with that of four chemical antioxidant activity assays, namely, DPPH radical scavenging, hydroxyl radical scavenging (HRS), reducing power (RP), and inhibition of linoleic acid peroxidation (ILAP). Results of the CAA assay were associated with those of DPPH and ILAP assays, but not with those of RP and HRS assays. The inter- and intra-specific antioxidant activities of CFS were characterized by chemical and CAA assays. L. rhamnosus CCFM 1107 displayed a high antioxidative effect similar to positive control L. rhamnosus GG ATCC 53103 in all of the assays. The CAA assay is a potential method for the detection of antioxidant activities of lactobacilli CFSs.

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Citations

Dec 3, 2016·Probiotics and Antimicrobial Proteins·Eric Banan-Mwine DaliriDeog H Oh
Mar 10, 2017·Probiotics and Antimicrobial Proteins·Anshul SharmaGoutam Rath
Jan 26, 2018·International Journal of Molecular Sciences·Weishun TianByung-Yong Park
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Jan 19, 2018·World Journal of Microbiology & Biotechnology·Maria MarsovaValeriy Danilenko
Feb 16, 2018·Probiotics and Antimicrobial Proteins·Zhenhui CaoZhiyong Zhao
Apr 15, 2018·Probiotics and Antimicrobial Proteins·Lvchen XiongDong Zeng
Dec 14, 2018·Probiotics and Antimicrobial Proteins·Tugce OnbasFadime Kiran
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Oct 13, 2020·Frontiers in Microbiology·Viviane Lima BatistaMariana Martins Drumond
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Jan 23, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ali OsmanMahmoud Sitohy

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Methods Mentioned

BETA
Assay

Software Mentioned

SPSS

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