Determining bacterial and host contributions to the human salivary metabolome.

Journal of Oral Microbiology
Alexander GardnerGuy H Carpenter

Abstract

Salivary metabolomics is rapidly advancing. To determine the extent to which salivary metabolites reflects host or microbial metabolic activity whole-mouth saliva (WMS), parotid saliva (PS) and plasma collected contemporaneously from healthy volunteers were analysed by 1H-NMR spectroscopy. Spectra underwent principal component analysis and k-means cluster analysis and metabolite quantification. WMS samples were cultured on both sucrose and peptide-enriched media. Correlation between metabolite concentration and bacterial load was assessed. WMS contained abundant short-chain fatty acids (SCFAs), which were minimal in PS and plasma. WMS spectral exhibited greater inter-individual variation than those of PS or plasma (6.7 and 3.6 fold, respectively), likely reflecting diversity of microbial metabolomes. WMS bacterial load correlated strongly with SCFA levels. Additional WMS metabolites including amines, amino acids and organic acids were positively correlated with bacterial load. Lactate, urea and citrate appeared to enter WMS via PS and the circulation. Urea correlated inversely with WMS bacterial load. Oral microbiota contribute significantly to the WMS metabolome. Several WMS metabolites (lactate, urea and citrate) are derived ...Continue Reading

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Citations

Jan 3, 2021·Metabolomics : Official Journal of the Metabolomic Society·Giacomo BaimaMario Aimetti
Feb 9, 2021·Frontiers in Microbiology·Leanne M CleaverGuy H Carpenter
May 26, 2021·NPJ Biofilms and Microbiomes·M Fata Moradali, Mary E Davey
Aug 13, 2020·Metabolites·Marco MeletiThelma A Pertinhez
Mar 26, 2021·Frontiers in Microbiology·Carla CuginiDaniel H Fine
Oct 2, 2020·Metabolomics : Official Journal of the Metabolomic Society·Jéssica Gardone VitórioRicardo Santiago Gomez
Nov 24, 2020·Periodontology 2000·Bhumika ShokeenRenate Lux
Mar 13, 2020·Metabolomics : Official Journal of the Metabolomic Society·Eric NeyraudCarole Tournier
Apr 15, 2020·Journal of Dental Research·G H Carpenter
May 21, 2020·Journal of Dental Research·A GardnerG H Carpenter
Jul 23, 2021·Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry·Angela RoveraPaul Anderson

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Software Mentioned

SPSS
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KNIME
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