Determining integrity of bladder innervation and smooth muscle function 1 year after lower spinal root transection in canines

Neurourology and Urodynamics
Danielle M SalvadeoMichael R Ruggieri

Abstract

To assess bladder smooth muscle function and innervation after long-term lower spinal root transection in canines. Thirteen female mixed-breed hound dogs underwent bladder decentralization, which included transection of all sacral dorsal and ventral roots caudal to L7 and hypogastric nerves, bilaterally (n = 3); all sacral roots and hypogastric nerves plus transection of L7 dorsal roots, bilaterally (n = 4); or a sham operation (n = 6). At a year after initial surgery, bladder function was assessed in vivo by stimulation of the pelvic plexus. The bladder tissue was harvested for ex vivo smooth muscle contractility studies. Remaining bladder was evaluated for nerve morphology immunohistochemically using neuronal marker PGP9.5, apoptotic activity using terminal deoxynucleotidyl transferase dUTP nick end labeling, and histopathology using a hematoxylin and eosin stain. Sacral root decentralization did not reduce maximum strength of pelvic plexus stimulation-induced bladder contraction, although long-term sacral dorsal and ventral root plus L7 dorsal root transection significantly decreased contraction strength. Electric field stimulation-induced contractions of the detrusor from all decentralized animals were preserved, compared t...Continue Reading

References

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Mar 27, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Mary F BarbeMichael R Ruggieri

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Citations

Jun 19, 2020·Npj Regenerative Medicine·Suradip DasD Kacy Cullen
Feb 14, 2021·Npj Regenerative Medicine·Suradip DasD Kacy Cullen
Mar 25, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nagat FraraMichael R Ruggieri

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