Determining major genotypes of hepatitis C virus among transplant recipients by real-time polymerase chain reaction assay

Jundishapur Journal of Microbiology
Roya FeyznezhadMazyar Ziyaeyan

Abstract

Hepatitis C virus (HCV) infection still exists as a health concern among the transplant patients. Because of the severity of the disease, different responses to treatment, and side effects resulting from long therapeutic period, determination of genotypes and viral loads can help choose the best treatment protocols. This study aimed to determine the HCV genotypes and its distribution patterns among liver, kidney, and bone marrow recipient candidates across Iran, referred to Namazi Hospital, southern Iran. A total of 101 individuals, including 44 (43.6%) liver, 55 (54.5%) kidney, and 2 (2%) bone marrow recipient candidates, with ages ranging between 5 and 74 years (Mean ±SD: 46.53 ± 13.73 y) participated in this study. From those, whole blood sample were collected and anti-HCV antibodies, RNA detection, and genotyping were performed on plasma using commercial chromatographic immunoassay, TaqMan one-step real-time polymerase chain reaction (RT-PCR), and genotyping RT-PCR kits, respectively. The frequencies of anti-HCV antibodies, RNA, various genotypes, and the viral load were compared with respect to gender, age, and transplant recipient groups. Of 101 individuals, 47 (46.5%) were positive for anti-HCV antibodies and 34 (33.7%) ...Continue Reading

References

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Citations

Feb 11, 2016·Jundishapur Journal of Microbiology·Mohammad Amin BehzadiMazyar Ziyaeyan
Dec 5, 2019·BMC Infectious Diseases·Mohammad Amin BehzadiMazyar Ziyaeyan

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Methods Mentioned

BETA
nucleic
PCR
Transplant
genotyping

Software Mentioned

SPSS

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