Determining mechanical features of modulated epithelial monolayers using subnuclear particle tracking

Journal of Cell Science
Travis J ArmigerKris Noel Dahl

Abstract

Force generation within cells, mediated by motor proteins along cytoskeletal networks, maintains the function of multicellular structures during homeostasis and when generating collective forces. Here, we describe the use of chromatin dynamics to detect cellular force propagation [a technique termed SINK (sensors from intranuclear kinetics)] and investigate the force response of cells to disruption of the monolayer and changes in substrate stiffness. We find that chromatin dynamics change in a substrate stiffness-dependent manner within epithelial monolayers. We also investigate point defects within monolayers to map the impact on the strain field of a heterogeneous monolayer. We find that cell monolayers behave as a colloidal assembly rather than as a continuum since the data fit an exponential decay; the lateral characteristic length of recovery from the mechanical defect is ∼50 µm for cells with a 10 µm spacing. At distances greater than this characteristic length, cells behave similarly to those in a fully intact monolayer. This work demonstrates the power of SINK to investigate diseases including cancer and atherosclerosis that result from single cells or heterogeneities in monolayers.This article has an associated First P...Continue Reading

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Citations

Sep 4, 2020·Journal of Cell Science·Jacob A VanderBurghCynthia A Reinhart-King
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Jian ZhangCynthia A Reinhart-King
Mar 20, 2021·Physical Review. E·Yan-Wei LiMassimo Pica Ciamarra
Apr 1, 2021·Molecular Biology of the Cell·Kirill LavrenyukKris Noel Dahl
Apr 11, 2021·Cytoskeleton·Maria Teresa ParreiraKris Noel Dahl

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